Anti-CD4 antibody [EDU-2] (FITC) (ab1154)

Overview

  • Product nameAnti-CD4 antibody [EDU-2] (FITC)
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [EDU-2] to CD4 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human T-lymphocytes.

  • General notesAb91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab1154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes1. Counting and identification of the T-helper/inducer lymphocyte subset in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
    2. Studies of HIV infection. Patients infected with HIV lose CD4+ and contemporarilyincrease CD8+ lymphocyte subset
    3. Characterization of subtypes of T-cell leukaemias and lymphomas.

    CD4 (FITC) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl ofCD4 (FITC) is sufficient for labelling of 1x106 cells.
    Molecular F/P ratio: between 3.0 and 5.0
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 Antigen antibody
      • CD4 molecule antibody
      • CD4 Receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T Cell Antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T Cell Surface Antigen T4/Leu3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T Cell Surface Glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    References for Anti-CD4 antibody [EDU-2] (FITC) (ab1154)

    ab1154 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry and patience. Ab18281 is supplied in a 2ml size and has been tested for application in FACS with human blood cells (it is recommended to use 20µl for 100µl whole human blood cells). Ab1154 is supplied in a 500 ul size and...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"