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Our Abpromise guarantee covers the use of ab1246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells. CD4 immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
Ab18414-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.
1. Counting and identification of the T-helper/inducer lymphocyte subset in human lysed whole peripheral blood or mononuclear cells separated by density gradient. 2. Studies of HIV infection. Patients infected with HIV lose CD4+ and contemporarilyincrease CD8+ lymphocyte subset 3. Characterization of subtypes of T-cell leukaemias and lymphomas.
ab1246 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"