You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-CD4 antibody [GK1.5] (ab25475)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-CD4 antibody [GK1.5]
See all CD4 products (97) ...

Description

Rat monoclonal [GK1.5] to CD4

Specificity

ab25475 recognises CD4/L3T4, Mr 52 kDa.

Tested applications

Functional Studies, Flow Cyt, IP, IHC-Frmore details

Cross reactivity

Reacts with

Mouse

Immunogen

Tissue / cell preparation (Mouse) - cloned mouse CTL line V4.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 100mM Borate buffered saline, pH 8.2

Concentration

Concentration information loading...

Purity

IgG fraction

Primary antibody notes

This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

Clonality

Monoclonal

Clone number

GK1.5

Isotype

IgG2b

Light chain type

kappa

Research areas

Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD

Applications

Show applications key

Our Abpromise guarantee covers the use of ab25475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    Functional Studies

     FuncS: Use at an assay d...Read more →

    FuncS: Use at an assay dependent dilution. (PubMed: 6415170(In vivo and in vitro depletion of CD4+ cells; Inhibition of T helper function and Fractionation of CD4+ cells) Use at an assay dependent dilution. For details see: Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1. See also PubMed: 6415170. For details see: Kruisbeek, A. M., et al.)

  • ShowHide

    Flow Cyt

     Flow Cyt: Use at an assa...Read more →

    Flow Cyt: Use at an assay dependent dilution.

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

  • ShowHide1 Image

    IHC-Fr

    Four stars (4 Abreviews) IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent concentration. (PubMed: 21345609)

Target

Function

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

Sequence similarities

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Post-translational
modifications

Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

Cellular localization

Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.

Target information above from: UniProt accessionP01730 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CD 4 antibody
  • CD4 (L3T4) antibody
  • CD4 antibody
  • CD4 antibody
  • CD4 antigen (p55) antibody
  • CD4 Antigen antibody
  • CD4 molecule antibody
  • CD4 Receptor antibody
  • CD4+ Lymphocyte deficiency, included antibody
  • CD4_HUMAN antibody
  • CD4mut antibody
  • L3T4 antibody
  • Leu3 antibody
  • Ly-4 antibody
  • Lymphocyte antigen CD4 antibody
  • MGC165891 antibody
  • OTTHUMP00000238897 antibody
  • p55 antibody
  • T Cell Antigen T4 antibody
  • T cell antigen T4/LEU3 antibody
  • T cell differentiation antigen L3T4 antibody
  • T cell OKT4 deficiency, included antibody
  • T cell surface antigen T4/Leu 3 antibody
  • T cell surface antigen T4/Leu3 antibody
  • T Cell Surface Antigen T4/Leu3 antibody
  • T Cell Surface Glycoprotein CD4 antibody
  • T-cell surface antigen T4/Leu-3 antibody
  • T-cell surface glycoprotein CD4 antibody
  • W3/25 antibody
  • W3/25 antigen antibody
see all

Database links

References for Anti-CD4 antibody [GK1.5] (ab25475)

This product has been referenced in:

  • Billiard Fet al. Abdominal ?-radiation induces an accumulation of function-impaired regulatory T cells in the small intestine. Int J Radiat Oncol Biol Phys 80:869-76 (2011). IHC-Fr; Mouse.Read more (PubMed: 21345609) »
  • Bursen Aet al. The AF4.MLL fusion protein is capable of inducing ALL in mice without requirement of MLL.AF4. Blood 115:3570-9 (2010). Flow Cyt; Mouse.Read more (PubMed: 20194896) »

See all 3 publications for this product

Publishing research using ab25475? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"