Anti-CD4 antibody [MEM-115] (ab91003)

Overview

  • Product nameAnti-CD4 antibody [MEM-115]
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [MEM-115] to CD4
  • Tested applicationsIHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    CD4 isolated from Human thymocytes and T lymphocytes

  • Epitopeab91003 recognizes an epitope in the D1 domain of the CD4 antigen.
  • Positive control
    • Human tonsil
  • General notesDilute immediately before use with PBS or Tris HCl.


    ab91003 is useful for the determination of the T4/T8 ratio in peripheral blood specimens and immunophenotyping of malignant T cell lymphomas and is an important tool for the in situ analysis of T cell subpopulations in infectious diseases and autoimmune conditions. ab91003 blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions. The MEM-115 clone blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions.

Properties

Applications

Our Abpromise guarantee covers the use of ab91003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/10.
Flow Cyt 1/10.
  • Application notesIs unsuitable for IHC-P or WB.
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu-3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    References for Anti-CD4 antibody [MEM-115] (ab91003)

    ab91003 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Resin sections)
    Sample Human Tissue sections (Nasal Polyp and Tonsil)
    Specification Nasal Polyp and Tonsil
    Username

    Abcam user community

    Verified customer

    Submitted Aug 24 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"