Anti-CD4 antibody [MEM-241] (PerCP) (ab65951)

Overview

  • Product nameAnti-CD4 antibody [MEM-241] (PerCP)See all CD4 primary antibodies ...
  • Description
    Mouse monoclonal [MEM-241] to CD4 (PerCP)
  • ConjugationPerCP. Ex: 482nm, Em: 675nm
  • Conjugation notesThe purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Human, Cynomolgus Monkey

    Does not react with

    Mouse
  • Immunogen

    Two N terminal domains of human CD4 fused to human IgG1 Fc

Properties

Applications

Our Abpromise guarantee covers the use of ab65951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
  • Application notesFlow Cyt: Use Neat: 10µl for 106 cells or 100µl of whole blood


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Target information above from: UniProt accession P01730 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Anti-CD4 antibody [MEM-241] (PerCP) images

    • Flow cytometry analysis of Human whole blood after red blod cell lysis, staining CD4 with ab65951. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.

      Gating Strategy: Live lymphocytes

      See Abreview

    • Flow cytometry analysis of Cynomolgus monkey whole blood after red blod cell lysis, staining CD4 with ab65951. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.

      Gating Strategy: Live lymphocytes

      See Abreview

    References for Anti-CD4 antibody [MEM-241] (PerCP) (ab65951)

    This product has been referenced in:
    • Bastien JP  et al. Photodepletion differentially affects CD4+ Tregs versus CD4+ effector T cells from patients with chronic graft-versus-host disease. Blood 116:4859-69 (2010). Flow Cyt ; Human . Read more (PubMed: 20798236) »

    See 1 Publication for this product

    Product Wall

    Application Flow Cytometry
    Sample Rat Cell (Splenocytes)
    Specification Splenocytes
    Preparation Cell harvesting/tissue preparation method: isolated from spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization No
    Gating Strategy alive lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Jan 09 2013

    Application Flow Cytometry
    Sample Mouse Cell (Splenocytes)
    Specification Splenocytes
    Preparation Cell harvesting/tissue preparation method: isolated from spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization No
    Gating Strategy alive lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Application Flow Cytometry
    Sample Cynomolgus Monkey Cell (from whole blood)
    Specification from whole blood
    Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy alive lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Application Flow Cytometry
    Sample Human Cell (from whole blood)
    Specification from whole blood
    Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy alive lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Thank you for contacting us. The lab has confirmed that this antibody is indeed specific to CD4. We will guarantee it not to react with any human IgG1 that may be present in your experiments. I hope this helps, please let me know if you need any additi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"