Anti-CD4 antibody [MEM-241] (Phycoerythrin) (ab18282)

Overview

  • Product nameAnti-CD4 antibody [MEM-241] (Phycoerythrin)See all CD4 primary antibodies ...
  • Description
    Mouse monoclonal [MEM-241] to CD4 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Conjugation notesThe purified antibody is conjugated with R-Phycoerythrin (R-PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    CD4 Fusion protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab18282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution. Use 5-20µl for 100µl whole human blood cells.

Target

  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 Antigen antibody
    • CD4 molecule antibody
    • CD4 Receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T Cell Antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T Cell Surface Antigen T4/Leu3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T Cell Surface Glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [MEM-241] (Phycoerythrin) images

  • ab18282 staining CD4 in human T cells by Flow Cytometry . 

References for Anti-CD4 antibody [MEM-241] (Phycoerythrin) (ab18282)

ab18282 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Permeabilization No
Sample Marmoset (common) Cell (blood)
Specification blood
Gating Strategy Live lymphocytes
Preparation Cell harvesting/tissue preparation method: red blood cell lysis
Sample buffer: PBS/BSA 0.5%
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Submitted Dec 20 2013

Application Flow Cytometry
Permeabilization No
Sample Mouse Cell (splenocytes)
Specification splenocytes
Gating Strategy alive lymphocytes
Preparation Cell harvesting/tissue preparation method: mechanical disruption
Sample buffer: PBS/BSA 0.5%
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Submitted Dec 19 2013

Application Flow Cytometry
Permeabilization No
Sample Cynomolgus Monkey Cell (blood)
Specification blood
Gating Strategy alive lymphocytes
Preparation Cell harvesting/tissue preparation method: red blood cell lysis
Sample buffer: PBS/BSA 0.5%
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Submitted Dec 19 2013

Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: Ficoll
Sample buffer: PBS+2%FCS
Fixation no fixation was performed
Permeabilization No
Gating Strategy alive cells
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Submitted Jan 14 2013

Application Flow Cytometry
Sample Monkey Cell (cells from blood)
Specification cells from blood
Preparation Cell harvesting/tissue preparation method: erythrocyte lysis
Sample buffer: PBS+2%FCS
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive cells
Username

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Submitted Jan 04 2013

Thank you so much for the additional information.
Yes, this probably explains why you did not get any signal with mouse and rat samples, but got signal with monkey.
Thank you so much for submitting your reviews!

Thank you for submitting your Abreviews for ab18282. As you may have noticed, your reviews has now been published on our website.
Since you obtained poor results using the antibody in the untested species (mouse and rat), we would like to follow up...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: harvesting of spleen
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy alive lymphocytes
Username

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Verified customer

Submitted Nov 13 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: harvesting of spleen
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Nov 13 2012

Application Flow Cytometry
Sample Monkey Cell (leucocytes from whole blood)
Specification leucocytes from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Nov 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"