![Flow Cytometry - CD4 antibody [RM4-5] (ab64144) image](#ab64144_1.jpg)
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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-CD4 antibody [RM4-5]
See all CD4 products (97) ...
Description
Rat monoclonal [RM4-5] to CD4
Tested applications
IHC-Fr, Flow Cytmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Tissue / cell preparation (Mouse): BALB/c mouse thymocytes.
Positive control
C57BL/6 mouse splenocytes.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Primary antibody notes
This antibody can be used for: Blocking of ligand binding and In vivo depletion of CD4+ cells.
Clonality
Monoclonal
Clone number
RM4-5
Isotype
IgG2a
Light chain type
kappa
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
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Flow Cytometry - CD4 antibody [RM4-5] (ab64144)
![Flow Cytometry - CD4 antibody [RM4-5] (ab64144) image](/ps/datasheet/Images/64/ab64144/ab64144_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab64144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution. (Fix with acetone.)
Flow Cyt: Use 0.25µg for 106 cells.
Target
Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Target information above from: UniProt accessionP01730
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Database links
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
Anti-CD4 antibody [RM4-5] images:
Flow Cytometry - CD4 antibody [RM4-5] (ab64144)
C57BL/6 mouse splenocytes stained with purified RM4-5, followed by anti-rat IgG FITC.
References for Anti-CD4 antibody [RM4-5] (ab64144)
ab64144 has not yet been referenced specifically in any publications.
Publishing research using ab64144? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"