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Flow Cytometry - CD4 antibody [RM4-5] (ab64144)
![Flow Cytometry - CD4 antibody [RM4-5] (ab64144)](/ps/datasheet/images/ab64144_1.jpg)
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Product Name
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CD4 antibody [RM4-5]
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See all CD4 antibodies (102)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [RM4-5] to CD4
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Immunogen
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Tissue / cell preparation (Mouse): BALB/c mouse thymocytes.
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Reacts with
(species key)
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Ms
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Tested applications
(see key)
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Flow Cyt, IHC-Fr
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 0.25µg for 106 cells in 100ul volume.
IHC-Fr (acetone fixed): Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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C57BL/6 mouse splenocytes.
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Cellular localization
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Cell membrane; Single pass type I membrane protein.
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Research areas
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Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
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Relevance
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CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
This antibody can be used for: Blocking of ligand binding and In vivo depletion of CD4+ cells.
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CD4 antibody [RM4-5] (ab64144) |
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 12504 (Mouse)
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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RM4-5
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD4 antibody [RM4-5] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD4 antibody [RM4-5] images, references, products related to ab64144 and other tools.
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CD4 antibody [RM4-5] images:
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 Flow Cytometry - CD4 antibody [RM4-5] (ab64144)
C57BL/6 mouse splenocytes stained with purified RM4-5, followed by anti-rat IgG FITC.
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Search PubMed (MEDLINE) for references to CD4
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CD4 antibody [RM4-5] - more information
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Customer reviews (feedback) regarding CD4 antibody [RM4-5] |
Customer FAQs regarding CD4 antibody [RM4-5] |
Protocols for CD4 antibody [RM4-5] |
Price and availability of products related to CD4 antibody [RM4-5] |
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CD4 antibody [RM4-5] - related products:
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CD4 antibody [RM4-5] - other tools:
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Contact Abcam with a Technical Enquiry about CD4 antibody [RM4-5] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Flow Cytometry - CD4 antibody [RM4-5] (ab64144)](/index.html?datasheet=64144#ab64144_1.jpg)
