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Anti-CD4 antibody [RPA-T4] (Biotin) (ab34271)


  • Product nameAnti-CD4 antibody [RPA-T4] (Biotin)
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [RPA-T4] to CD4 (Biotin)
  • ConjugationBiotin
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human) -PHA blasts.

  • Epitopethis antibody recognises an epitope within domain 1, of the extracellular region, of the CD4 molecule.
  • Positive control
    • human peripheral blood lymphocytes
  • General notes

    This antibody has been reported to block gp120-CD4 interaction and inhibit syncytium formation.



Our Abpromise guarantee covers the use of ab34271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use neat - 1/10. Use 10µl of this dilution for 106 cells or 100µl whole blood.

ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [RPA-T4] (Biotin) images

  • FACS analysis of CD4 expressing cells in human peripheral blood lymphocytes using ab34271.

References for Anti-CD4 antibody [RPA-T4] (Biotin) (ab34271)

ab34271 has not yet been referenced specifically in any publications.

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Thanks for your call today and for your questions about our CD4 antibodies. Since neither ab28069 or ab34271 has been tested in a sandwich ELISA with ab51312, I can offer a discount off a future purchase if you buy one of these biotinylated CD4 ...

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