Anti-CD4 antibody [RPA-T4] (Biotin) (ab34271)

Overview

  • Product nameAnti-CD4 antibody [RPA-T4] (Biotin)See all CD4 primary antibodies ...
  • Description
    Mouse monoclonal [RPA-T4] to CD4 (Biotin)
  • ConjugationBiotin
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human) -PHA blasts.

  • Epitopethis antibody recognises an epitope within domain 1, of the extracellular region, of the CD4 molecule.
  • Positive control
    • human peripheral blood lymphocytes
  • General notes


    This antibody has been reported to block gp120-CD4 interaction and inhibit syncytium formation.

Properties

Applications

Our Abpromise guarantee covers the use of ab34271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
  • Application notesFlow Cyt: Use neat - 1/10. Use 10µl of this dilution for 106 cells or 100µl whole blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Target information above from: UniProt accession P01730 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 Antigen antibody
      • CD4 molecule antibody
      • CD4 Receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T Cell Antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T Cell Surface Antigen T4/Leu3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T Cell Surface Glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Anti-CD4 antibody [RPA-T4] (Biotin) images

    • FACS analysis of CD4 expressing cells in human peripheral blood lymphocytes using ab34271.

    References for Anti-CD4 antibody [RPA-T4] (Biotin) (ab34271)

    ab34271 has not yet been referenced specifically in any publications.

    Product Wall

    Thanks for your call today and for your questions about our CD4 antibodies. Since neither ab28069 or ab34271 has been tested in a sandwich ELISA with ab51312, I can offer a discount off a future purchase if you buy one of these biotinylated CD4 ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"