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2 questions for ab61131
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Question 1
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Friday 20-April-2012 |
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Searching for rabbit specific - anti CD3 - anti FOXP3 - anti CD4 - anti CD8 - anti CD1A antibodies |
ANSWER: |
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Thank you very much for your phone call.
The best antibodies we can recommend are as follows. Please note that the recommendations are based on immunogen sequence only we currently do not hold any rabbit specific data.
Anti CD3 : ab16669; I can provide Abreview image discount code and will also provide Abpromise guarantee on use for this ab. Anti FOXP3: ab20034, ab22510 are best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work. Anti CD4: ab61131, ab101530 and ab108329, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work. anti CD8: ab108343, ab123630 and ab86733, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.
Anti CD1A : ab76531, ab108309 and ab123133 are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.
Please also note that most of these antibodies are raised in Rabbit so there will be high back ground staining. Our recommendations to reduce the back ground staining are
- Use preadsorbed anti body e.g. ab7132, ab7083, ab
- Use (Fab)2 fragments antibodies e.g. ab6112, ab102296
- Incubate sections with affinity pure Fab fragment anti rabbit antibody before applying primary antibody
Please check the full details by clicking the links;
http://www.abcam.com/index.html?pageconfig=resource&rid=12722 http://www.abcam.com/index.html?pageconfig=resource&rid=12825 http://www.abcam.com/index.html?pageconfig=resource&rid=11465
The abreview discount code details can be found by clicking following link;
http://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
Could you please select the antibodies you are interested in, I will then be happy in creating a discount code for each one of these.
I will look forward to hearing from you soon. |
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Question 2
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Monday 16-January-2012 |
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thank you for your prompt reply. I could try and ab tested already for IHC in other species. Which one would you suggest me? Thank you very much. |
ANSWER: |
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Thank you very much for your interest in our products. Unfortunately, the homology between rabbit and human CD4 is not especially high (61%). But I am happy to confirm that the immunogen of ab61131 shows a 84% homology with rabbit. At this homology we can anticipate that the antibody might cross react. This antibody is already tested for IHC-P. But ab61131 has not been tested in rabbit. Therefore, I can offer a discount off a future purchase if you buy ab61131 now, test it inrabbit and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab61131 in rabbit. I will then send a discount code. This code must be issued before purchasing ab61131 so please wait for my reply before ordering. 2. Purchase ab61131 either by phone, fax, or online (www.abcam.com). 3. Test it in rabbit . 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab61131 turns out to be unsuitable for rabbit, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"