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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-CD4 antibody
See all CD4 products (97) ...
Description
Rabbit polyclonal to CD4
Specificity
ab61131 detects endogenous levels of total CD4 protein.
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic non-phosphopeptide derived from human CD4 around the phosphorylation site of serine 433.
Positive control
Human brain tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab61131 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody (ab61131)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
Target
Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Target information above from: UniProt accessionP01730
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
Anti-CD4 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody (ab61131)
ab61131 at 1/50 dilution staining CD4 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
References for Anti-CD4 antibody (ab61131)
ab61131 has not yet been referenced specifically in any publications.
Publishing research using ab61131? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"