|
|
|
|
Western blot - CD4 antibody (ab51312)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [mAb51312] (ab51312)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [mAb51312] (ab51312)](/ps/datasheet/images/ab51312_1 copy.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
CD4 antibody [mAb51312]
|
| |
See all CD4 antibodies (102)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [mAb51312] to CD4
|
|
Immunogen
|
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human CD4. (Peptide available as ab54699.)
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Tested applications
(see key)
|
IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: Use at a dilution of 1/50 for 1 hr.
WB: Use at a concentration of 8 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
This antibody gave a positive signal in Human Thymus and Human Lymph Node Tissue Lysate
|
|
Cellular localization
|
Cell membrane; Single pass type I membrane protein.
|
|
Research areas
|
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
|
|
Relevance
|
CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
|
|
|
CD4 antibody [mAb51312] (ab51312) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
mAb51312
|
|
Isotype
|
IgG1
|
|
Light chain type
|
kappa
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD4 antibody [mAb51312] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for CD4 antibody [mAb51312] images, references, products related to ab51312 and other tools.
|
| |
CD4 antibody [mAb51312] images:
|
|
|
| |
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [mAb51312] (ab51312)
ab51312 staining CD4 in mouse brain tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxidase block and 10 minutes for protein block). The primary antibody was diluted 1/250 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to mouse IgG was used undiluted as secondary. This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
See Abreview
|
| |
| |
|
Search PubMed (MEDLINE) for references to CD4
|
| |
CD4 antibody [mAb51312] - more information
|
Customer reviews (feedback) regarding CD4 antibody [mAb51312] |
Customer FAQs regarding CD4 antibody [mAb51312] |
Protocols for CD4 antibody [mAb51312] |
Price and availability of products related to CD4 antibody [mAb51312] |
| |
CD4 antibody [mAb51312] - related products:
|
|
|
| |
CD4 antibody [mAb51312] - other tools:
|
Contact Abcam with a Technical Enquiry about CD4 antibody [mAb51312] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [mAb51312] (ab51312)](/index.html?datasheet=51312#ab51312_1 copy.jpg)
