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ab54699 |
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ab51312 has been referenced in 4 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab51312 staining CD4 in mouse brain tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxidase block and 10 minutes for protein block). The primary antibody was diluted 1/250 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to mouse IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
All lanes : Anti-CD4 antibody [mAb51312] (ab51312) at 5 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 :
Lane 3 :
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 51 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
6
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