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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human CD4.
(Peptide available as ab54699.)
Our Abpromise guarantee covers the use of ab67480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 8 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).Can be blocked with Human CD4 peptide (ab54699).|
ab67480 staining CD4 in human tonsil tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in target retrieval solution and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to mouse was used undiluted as the secondary.
ab67480 has not yet been referenced specifically in any publications.