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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-CD4 (phospho S433) antibody
See all CD4 products (97) ...
Description
Rabbit polyclonal to CD4 (phospho S433)
Specificity
ab61011 detects endogenous levels of CD4 only when phosphorylated at serine 433.
Tested applications
IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from human CD4 around the phosphorylation site of serine 433 (R-M-SP-Q-I).
Positive control
Human brain tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab61011 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 (phospho S433) antibody (ab61011)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/40000ELISA: 1/40000
Target
Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Target information above from: UniProt accessionP01730
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
Anti-CD4 (phospho S433) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 (phospho S433) antibody (ab61011)
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab61011 at a 1/50 dilution.
References for Anti-CD4 (phospho S433) antibody (ab61011)
ab61011 has not yet been referenced specifically in any publications.
Publishing research using ab61011? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"