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Overview

  • Product nameCD40 Human ELISA KitSee all CD40 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 50 pg/ml
  • Range
    54.87 pg/ml - 40000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 92.27 80% - 100%
    Serum 114.7 104% - 124%
    Plasma 90.19 78% - 100%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab99990 Human CD40 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD40 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    500X HRP-Streptavidin Concentrate Item G 1 x 200µl
    Assay Diluent B 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent. 1 x 15ml
    Assay Diluent D 5x concentrated buffer. For Standard/Sample (serum/plasma samples/ cell culture medium/urine) diluent. 1 x 15ml
    CD40 Microplate (12 strips x 8 wells) coated with anti-human CD40 1 unit
    CD40 Standard Human(recombinant) 2 vials
    Detection Antibody CD40: biotinylated anti-human CD40 2 vials
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • RelevanceCD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
    • Alternative names
        AI326936B cell associated molecule B cell associated molecule CD40
        B cell surface antigen CD40 Bp50CD 40 CD40 antigen CD40 antigen (TNF receptor superfamily member 5)CD40 molecule CD40 molecule TNF receptor superfamily member 5CD40 protein CD40 type II isoform CD40L receptor CDw40GP39 HIGM1IGM MGC9013Nerve growth factor receptor related B lymphocyte activation molecule NFRSF5 OTTHUMP00000031699OTTHUMP00000031700 p50T BAM TBAMTNF receptor superfamily member 5TNFRSF5TRAPTumor necrosis factor receptor superfamily member 5Tumor necrosis factor receptor superfamily member 5 precursor Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab99990 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      CD40 Human ELISA Kit images

      • ELISA - CD40 Human ELISA Kit (ab99990)
        ELISA - CD40 Human ELISA Kit (ab99990)

      Protocols

      References for CD40 Human ELISA Kit (ab99990)

      ab99990 has not yet been referenced specifically in any publications.

      Publishing research using ab99990 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab99990 Submit a Question for ab99990

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      Has anybody tried to use this human kit to measure dog CD40?

      Thank you very much for your interest in ab99990.


      To our knowledge, ab99990 has not been tested in canine samples. Overall, there is only about 70% homology with the human and canine sequences. Therefore, I can offer a discount off a futu...

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      Is the antibody that is coated to the plate of this kit commercially available?

      Thank you for your inquiry.


      So I received a fairly cryptic reply from the lab regarding your inquiry. They said that upon request, they can sell antibodies from their kits separately, but since these are special requests, there won't be a ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"