CD40 Human ELISA Kit (ab99990)
- Product nameCD40 Human ELISA KitSee all CD40 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 50 pg/ml
- Range54.87 pg/ml - 40000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 92.27 80% - 100% Serum 114.7 104% - 124% Plasma 90.19 78% - 100%
- Species reactivityReacts with: Human
- Product overview
ab99990 Human CD40 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD40 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 500X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent B 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent. 1 x 15ml Assay Diluent D 5x concentrated buffer. For Standard/Sample (serum/plasma samples/ cell culture medium/urine) diluent. 1 x 15ml CD40 Microplate (12 strips x 8 wells) coated with anti-human CD40 1 unit CD40 Standard Human(recombinant) 2 vials Detection Antibody CD40: biotinylated anti-human CD40 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- AI326936B cell associated molecule B cell associated molecule CD40
- B cell surface antigen CD40 Bp50CD 40 CD40 antigen CD40 antigen (TNF receptor superfamily member 5)CD40 molecule CD40 molecule TNF receptor superfamily member 5CD40 protein CD40 type II isoform CD40L receptor CDw40GP39 HIGM1IGM MGC9013Nerve growth factor receptor related B lymphocyte activation molecule NFRSF5 OTTHUMP00000031699OTTHUMP00000031700 p50T BAM TBAMTNF receptor superfamily member 5TNFRSF5TRAPTumor necrosis factor receptor superfamily member 5Tumor necrosis factor receptor superfamily member 5 precursor Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a
Our Abpromise guarantee covers the use of ab99990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for CD40 Human ELISA Kit (ab99990)
ab99990 has not yet been referenced specifically in any publications.