The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5-1µg for 105-8 cells. Staining the cell sample in a final volume of 100µl is recommended.
FunctionReceptor for TNFSF5/CD40LG.
Tissue specificityB-cells and in primary carcinomas.
Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
CD40 antigen (TNF receptor superfamily member 5) antibody
CD40 antigen antibody
CD40 molecule antibody
CD40 molecule, TNF receptor superfamily member 5 antibody
CD40 protein antibody
CD40 type II isoform antibody
CD40L receptor antibody
Nerve growth factor receptor related B lymphocyte activation molecule antibody
TNF receptor superfamily member 5 antibody
Tumor necrosis factor receptor superfamily , member 5 antibody
Tumor necrosis factor receptor superfamily member 5 antibody
Tumor necrosis factor receptor superfamily member 5 precursor antibody
Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
Anti-CD40 antibody [HM40-3] images
Flow Cytometry - CD40 antibody [HM40-3] (ab95533)
Flow cytometry staining of BALB/c splenocytes with 0.5 µg Armenian Hamster IgG Isotype Control (open histogram) or 0.5 µg ab95533 (filled histogram) followed by Anti-Armenian Hamster IgG FITC. Total viable cells were used for analysis.
References for Anti-CD40 antibody [HM40-3] (ab95533)
This product has been referenced in:
Inaba K et al. The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J Exp Med180:1849-60 (1994).
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