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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - CD40 antibody (ab13545)
All lanes : Anti-CD40 antibody (ab13545) at 1/1000 dilution
Lane 1 : Control human HCAEC cells - whole cell lysate
Lane 2 : Control human HCAEC cells - whole cell lysate
Lane 3 : Treated human HCAEC cells - whole cell lysate
Lane 4 : Treated human HCAEC cells - whole cell lysate
Secondary
HRP conjugated Goat Anti-Rabbit IgG
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 34 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 seconds
This image is courtesy of an Abreview submitted on 10 August 2005.
Western blot - CD40 antibody (ab13545)
All lanes : Anti-CD40 antibody (ab13545) at 1/5000 dilution
Lane 1 : Human B chronic lymphocytic leukaemia cells, nuclear fraction
Lane 2 : Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction
Lane 3 : Human B chronic lymphocytic leukaemia cells, membrane fraction
Lane 4 : Human B chronic lymphocytic leukaemia cells, cytosolic fraction
Lysates/proteins at 18 µg per lane.
Secondary
HRP conjugated goat anti-rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 34 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
The primary antibody was incubated with the membrane for 16 hours at 4°C.
This image is courtesy of an anonymous Abreview
Western blot
Anti-CD40 antibody (ab13545) at 1/1000 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 8 minutes
CD40 antibody for WB in Human (13545)
CD40 antibody for WB in Human (13545)
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