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Products:Immunology >> Adaptive Immunity >> B Cells >> CD
Overview
Product name
Anti-CD40 antibody
See all CD40 products (42) ...
Description
Rabbit polyclonal to CD40
Specificity
The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Monkey
Predicted to work with
Chicken, Dog
Immunogen
Synthetic peptide: CQPVTQEDGKESRISVQERQ conjugated to KLH, corresponding to amino acids 258-277 of Human CD40.
CQPVTQEDGK ESRISVQERQ
Positive control
HeLa Cell Lysate.
General notes
Storage below -20°C is not recommended.
For maximum product recovery centrifuge the
product vial before removing cap. Shipped on gel packs.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS, 0.05mM PMSF.
Purity
Whole antiserum
Purification notes
Purified on a peptide bound immunoaffinity column.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Innate and adaptive immunity
Immunology >> Adaptive Immunity >> B Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
Target
Function
Receptor for TNFSF5/CD40LG.
Tissue specificity
B-cells and in primary carcinomas.
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Sequence similarities
Contains 4 TNFR-Cys repeats.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP25942
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Tumor necrosis factor receptor superfamily , member 5 antibody
- AI326936 antibody
- B cell associated molecule CD40 antibody
see all
Database links
- Entrez Gene: 395385 Chicken
- Entrez Gene: 403469 Dog
- Entrez Gene: 958 Human
- Entrez Gene: 21939 Mouse
- Entrez Gene: 171369 Rat
- Omim: 109535 Human
- SwissProt: Q7YRL5 Dog
- SwissProt: P25942 Human
see all
Anti-CD40 antibody images:
Western blot - CD40 antibody (ab13545)
All lanes : Anti-CD40 antibody (ab13545) at 1/1000 dilution
Lane 1 : Control human HCAEC cells - whole cell lysate
Lane 2 : Control human HCAEC cells - whole cell lysate
Lane 3 : Treated human HCAEC cells - whole cell lysate
Lane 4 : Treated human HCAEC cells - whole cell lysate
Secondary
HRP conjugated Goat Anti-Rabbit IgG
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 34 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 seconds
This image is courtesy of an Abreview submitted on 10 August 2005.
Western blot - CD40 antibody (ab13545)
All lanes : Anti-CD40 antibody (ab13545) at 1/5000 dilution
Lane 1 : Human B chronic lymphocytic leukaemia cells, nuclear fraction
Lane 2 : Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction
Lane 3 : Human B chronic lymphocytic leukaemia cells, membrane fraction
Lane 4 : Human B chronic lymphocytic leukaemia cells, cytosolic fraction
Lysates/proteins at 18 µg per lane.
Secondary
HRP conjugated goat anti-rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 34 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
The primary antibody was incubated with the membrane for 16 hours at 4°C.
This image is courtesy of an anonymous Abreview
Western blot
Anti-CD40 antibody (ab13545) at 1/1000 dilution + CD40 protein (Fc Chimera Active) (ab83917) at 1 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 8 minutes
References for Anti-CD40 antibody (ab13545)
This product has been referenced in:
- Twig Get al. Synergistic amplification of beta-amyloid- and interferon-gamma-induced microglial neurotoxic response by the senile plaque component chromogranin A. Am J Physiol Cell Physiol 288:C169-75 (2005).Read more (PubMed: 15342341) »
See 1 publication for this product
Publishing research using ab13545? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"