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Products:Immunology >> Adaptive Immunity >> B Cells >> CD
Overview
Product name
Anti-CD40 antibody
See all CD40 products (42) ...
Description
Rabbit polyclonal to CD40
Specificity
This antibody detects endogenous levels of total CD40 protein.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide derived from the C-terminal of CD40 (Human)
Positive control
COS7 cell extract (Western blot). Human breast carcinoma (IHC-P).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Innate and adaptive immunity
Immunology >> Adaptive Immunity >> B Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
Target
Function
Receptor for TNFSF5/CD40LG.
Tissue specificity
B-cells and in primary carcinomas.
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Sequence similarities
Contains 4 TNFR-Cys repeats.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP25942
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Tumor necrosis factor receptor superfamily , member 5 antibody
- AI326936 antibody
- B cell associated molecule CD40 antibody
see all
Database links
- Entrez Gene: 958 Human
- Entrez Gene: 21939 Mouse
- Omim: 109535 Human
- SwissProt: P25942 Human
- SwissProt: P27512 Mouse
- SwissProt: Q8K2X6 Mouse
- Unigene: 472860 Human
- Unigene: 271833 Mouse
Anti-CD40 antibody images:
Western blot - CD40 antibody (ab58391)
All lanes : Anti-CD40 antibody (ab58391) at 1/500 dilution
Lane 1 : COS7 cell extract with no immunizing peptide
Lane 2 : COS7 cell extract with immunizing peptide
Predicted band size : 31 kDa
Observed band size : 31 kDa
Immunohistochemistry (Paraffin-embedded sections) - CD40 antibody (ab58391)
ab58391, at 1/50 diltuion, staining human CD40 in breast carcinoma tissue, by immunohistochemistry, in the presence or absence of the immunizing peptide. Paraffin embedded tissue.
References for Anti-CD40 antibody (ab58391)
This product has been referenced in:
- Manczak Met al. Neutralization of granulocyte macrophage colony-stimulating factor decreases amyloid beta 1-42 and suppresses microglial activity in a transgenic mouse model of Alzheimer's disease. Hum Mol Genet 18:3876-93 (2009). IHC-Fr; Mouse.Read more (PubMed: 19617638) »
See 1 publication for this product
Publishing research using ab58391? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"