Anti-CD40 antibody (ab58612)

I am a researcher who is using anti-CD40 (ab58612) in immunocytochemical and immunohistochemical analyses.
I am using a CD40 knock-out mouse (cd40-/-) in which exon 3 of the cd40 gene has been targeted for disruption by a neomycin cassette (ref: Kawabe et al, 1994: http://www.sciencedirect.com/science/article/pii/1074761394900957).
I understand that the sequence of your immunising peptide is proprietary, however could you please inform me if this antibody should detect cd40 in these cd40-/- mice, i.e. does it bind any part of the protein that is encoded by exon 3 or downstream of exon 3?
This information is critical to validating this antibody for our use. I respect you cannot inform me of the sequence that the antibody is raised against, but if you
could tell me whether this antibody will detect cd40 in mouse cd40-/- tissues, I would be most grateful.
Additionally, If you could recommend an antibody that would be useful to detect CD40 in wild type tissues but should be negative in cd40-/- mice, this would be most helpful.

ANSWER:

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field.

I can, however, tell you the range in which you can find the epitopeof this antibody:The epitope is located within aa 230-277, Isoform I, of the human protein.

If you could provide me with the amino acid sequence, I'll be happy looking for a suitable antibody for you if this one should be of no use for you.

I hope this information is helpful to you.Please do not hesitate to contact us again

Could you please detail the exact predicted binding sites of the

following antibodies:

CD40 antibody: ab58612 (Rabbit polyclonal to CD40)

- Specifically, where in the C-terminus does this antibody bind -

which domain etc?

- Can you please issue the sequence of this immunizing peptide? Is it

available for purchase?

CD40L antibody: ab2391 (Hamster monoclonal to CD40L)

- Specifically, where in CD40L does this antibody bind?

- Can you please issue the sequence of this immunizing peptide?

- Additionally, would you predict that this antibody will recognize

mouse CD40L based on the immunizing sequence?

CD40L antibody: ab65854 (Rabbit polyclonal to CD40L)

- It states that it is raised against a synthetic peptide derived from

the N-terminus of human CD40L, but where in the N-terminus does this

antibody bind?

Can you please issue the sequence of this immunizing peptide?

CD40L antibody [MR-1]: ab99894 (Hamster monoclonal to CD40L)

- Specifically, which region of CD40L does this antibody interact with?

Additionally, can you recommend negative tissue type controls to use

for CD40 and CD40L?

I would greatly appreciate assistance

ANSWER:

Thank you for contacting Abcam regarding our CD40 and CD40L antibodies.

Below please find the requested information:

ab2391 Rabbit Polyclonal to CD40L - Unfortunately the specific immunogen sequence is proprietary, however I can inform you that it is a small peptide that surrounds aa246 of human CD40L. Also, I have learned from the laboratory that this antibody will react with mouse and is guaranteed to do so.

ab58612 Rabbit Polyclonal to CD40 - The specific sequence is proprietary. Unfortunately, the blocking peptide is not commercially available.

ab65854 Rabbit Polyclonal to CD40L - Please contact us for additional immunogen information.

ab99894 Hamster monoclonal to CD40L - Unfortunately the epitope has not been mapped for this antibody.

Regarding a possible negative control - for CD40 and CD40L I would recommend liver.

I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.