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Products:Immunology >> Adaptive Immunity >> B Cells >> CD
Overview
Product name
Anti-CD40 antibody
See all CD40 products (42) ...
Description
Rabbit polyclonal to CD40
Tested applications
ICC, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of Human CD40, identical to the related rat and mouse sequence.
Positive control
Hela Cell Lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 0.18% Sodium chloride, 0.04% Dibasic monohydrogen sodium phosphate, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Innate and adaptive immunity
Immunology >> Adaptive Immunity >> B Cells >> CD
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Western blot - CD40 antibody (ab65853)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use a concentration of 0.5 - 1 µg/ml.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 45 kDa (predicted molecular weight: 31 kDa).
Target
Function
Receptor for TNFSF5/CD40LG.
Tissue specificity
B-cells and in primary carcinomas.
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Sequence similarities
Contains 4 TNFR-Cys repeats.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP25942
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Tumor necrosis factor receptor superfamily , member 5 antibody
- AI326936 antibody
- B cell associated molecule CD40 antibody
see all
Database links
- Entrez Gene: 958 Human
- Entrez Gene: 21939 Mouse
- Entrez Gene: 171369 Rat
- Omim: 109535 Human
- SwissProt: P25942 Human
- SwissProt: P27512 Mouse
- SwissProt: Q8K2X6 Mouse
- SwissProt: Q4QQW2 Rat
see all
Anti-CD40 antibody images:
Western blot - CD40 antibody (ab65853)
Anti-CD40 antibody (ab65853) at 2 µg/ml + Hela cell lysate
Predicted band size : 31 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
References for Anti-CD40 antibody (ab65853)
ab65853 has not yet been referenced specifically in any publications.
Publishing research using ab65853? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"