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Anti-CD40 (phospho T254) antibody (ab79278)

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Overview

Product name

Anti-CD40 (phospho T254) antibody
See all CD40 products (42) ...

Description

Rabbit polyclonal to CD40 (phospho T254)

Specificity

ab79278 detects endogenous levels of CD40 only when phosphorylated at threonine 254 (Human: Thr254; Mouse: Thr255).

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic phosphopeptide derived from human CD40 around the phosphorylation site of threonine 254 (Q-E-TP-L-H).

Positive control

COLO cell extracts, treated with Insulin (0.01U/ml, 15mins).

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab79278 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Innate and adaptive immunity
Immunology >> Adaptive Immunity >> B Cells >> CD

  • Western blot - CD40 (phospho T254) antibody (ab79278)Western blot - CD40 (phospho T254) antibody (ab79278) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab79278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Predi...Read more →

    WB: 1/500 - 1/1000.Predicted molecular weight: 31 kDa.

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

Target

Function

Receptor for TNFSF5/CD40LG.

Tissue specificity

B-cells and in primary carcinomas.

Involvement in disease

Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.

Sequence similarities

Contains 4 TNFR-Cys repeats.

Cellular localization

Secreted and Cell membrane.

Target information above from: UniProt accessionP25942 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Tumor necrosis factor receptor superfamily , member 5 antibody
  • AI326936 antibody
  • B cell associated molecule CD40 antibody
  • B cell surface antigen CD40 antibody
  • B cell-associated molecule antibody
  • B-cell surface antigen CD40 antibody
  • Bp50 antibody
  • CD 40 antibody
  • CD40 antibody
  • CD40 antibody
  • CD40 antigen (TNF receptor superfamily member 5) antibody
  • CD40 antigen antibody
  • CD40 molecule antibody
  • CD40 molecule, TNF receptor superfamily member 5 antibody
  • CD40 protein antibody
  • CD40 type II isoform antibody
  • CD40L receptor antibody
  • CDw40 antibody
  • GP39 antibody
  • HIGM1 antibody
  • IGM antibody
  • IMD3 antibody
  • MGC9013 antibody
  • Nerve growth factor receptor related B lymphocyte activation molecule antibody
  • OTTHUMP00000031699 antibody
  • OTTHUMP00000031700 antibody
  • p50 antibody
  • T-BAM antibody
  • TBAM antibody
  • TNF receptor superfamily member 5 antibody
  • TNFRSF5 antibody
  • TNR5_HUMAN antibody
  • TRAP antibody
  • Tumor necrosis factor receptor superfamily member 5 antibody
  • Tumor necrosis factor receptor superfamily member 5 precursor antibody
  • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
see all

Database links

Anti-CD40 (phospho T254) antibody images:

  Western blot - CD40 (phospho T254) antibody (ab79278)

Western blot - CD40 (phospho T254) antibody (ab79278)

All lanes : Anti-CD40 (phospho T254) antibody (ab79278) at 1/500 dilution

Lane 1 : COLO cell extract, treated with Insulin (0.01U/ml, 15mins)
Lane 2 : COLO cell extract, treated with Insulin (0.01U/ml, 15mins) with immunising phosphopeptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 31 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)

References for Anti-CD40 (phospho T254) antibody (ab79278)

ab79278 has not yet been referenced specifically in any publications.

Publishing research using ab79278? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"