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Western blot - CD40 (phospho T254) antibody (ab79278)

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Product Name
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CD40 (phospho T254) antibody
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See all CD40 antibodies (38)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CD40 (phospho T254)
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Immunogen
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Synthetic phosphopeptide derived from human CD40 around the phosphorylation site of threonine 254 (Q-E-TP-L-H).
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms (Due to sequence homology)
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Specificity
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ab79278 detects endogenous levels of CD40 only when phosphorylated at threonine 254 (Human: Thr254; Mouse: Thr255).
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/5000. WB: 1/500 - 1/1000. Predicted molecular weight: 31 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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COLO cell extracts, treated with Insulin (0.01U/ml, 15mins).
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Cellular localization
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Isoform I: Cell membrane; Single-pass type I membrane protein. Isoform II: Secreted.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage Immunology >> Adaptive Immunity >> B Cells >> CD
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Relevance
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CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
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Purification notes
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ab79278 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD40 (phospho T254) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD40 (phospho T254) antibody images, references, products related to ab79278 and other tools.
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CD40 (phospho T254) antibody images:
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Western blot - CD40 (phospho T254) antibody (ab79278)
All lanes : CD40 (phospho T254) antibody (ab79278) at 1/500 dilution
Lane 1 : COLO cell extract, treated
with Insulin (0.01U/ml, 15mins) Lane 2 : COLO cell extract, treated
with Insulin (0.01U/ml, 15mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 31 kDa Observed band size : 26 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to CD40
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CD40 (phospho T254) antibody - more information
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Customer reviews (feedback) regarding CD40 (phospho T254) antibody |
Customer FAQs regarding CD40 (phospho T254) antibody |
Protocols for CD40 (phospho T254) antibody |
Price and availability of products related to CD40 (phospho T254) antibody |
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CD40 (phospho T254) antibody - related products:
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CD40 (phospho T254) antibody - other tools:
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Contact Abcam with a Technical Enquiry about CD40 (phospho T254) antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"