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Anti-CD40 (phospho T254) antibody
See all CD40 products (42) ...
Rabbit polyclonal to CD40 (phospho T254)
ab79278 detects endogenous levels of CD40 only when phosphorylated at threonine 254 (Human: Thr254; Mouse: Thr255).
WB, ELISAmore details
Reacts with
Human
Predicted to work with
Mouse
Synthetic phosphopeptide derived from human CD40 around the phosphorylation site of threonine 254 (Q-E-TP-L-H).
COLO cell extracts, treated with Insulin (0.01U/ml, 15mins).
Liquid
Store at -20°C. Stable for 12 months at -20°C
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab79278 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Polyclonal
IgG
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Innate and adaptive immunity
Immunology >> Adaptive Immunity >> B Cells >> CD
Western blot - CD40 (phospho T254) antibody (ab79278)
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Our Abpromise guarantee covers the use of ab79278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 31 kDa.
ELISA: 1/5000
Receptor for TNFSF5/CD40LG.
B-cells and in primary carcinomas.
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Contains 4 TNFR-Cys repeats.
Secreted and Cell membrane.
Target information above from: UniProt accessionP25942
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - CD40 (phospho T254) antibody (ab79278)

All lanes : Anti-CD40 (phospho T254) antibody (ab79278) at 1/500 dilution
Lane 1 : COLO cell extract, treated
with Insulin (0.01U/ml, 15mins)
Lane 2 : COLO cell extract, treated
with Insulin (0.01U/ml, 15mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 31 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
ab79278 has not yet been referenced specifically in any publications.
Publishing research using ab79278? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-CD40 (phospho T254) antibody (ab79278) at 1/500 dilution
Lane 1 : COLO cell extract, treated
with Insulin (0.01U/ml, 15mins)
Lane 2 : COLO cell extract, treated
with Insulin (0.01U/ml, 15mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 31 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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