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Overview

  • Product nameCD40 protein (Active)See all CD40 proteins and peptides ...
  • Protein descriptionRecombinant full length Human CD40 (extracellular domain amino acids 1–193) fused to the Fc region of Human IgG1 (aa 90-330) expressed in modified Human 293 cells. Swiss ID = P25942.
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityActivity: The ED50 of ab83917 is typically 1.0-1.5 ug/ml as measured by its ability to neutralize CD40 ligand mediated proliferation of T47-D cells.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: EPPTACREKQYLINSQCCSLCQPGQKLVS DCTEFTETECLPCGESEFLDTWN RETHCHQHKYCDPNL GLRVQQKGTSETDTICTCEEGWHCTSEACESCVLHR SC SPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWT SCETKDLVVQ QAGTNKTDVVCGPQDRLRRSSNTKVDKK VEPKSCDKTHTCPPCPAPELLGG PSVFLFPPKPKDTLM ISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTK PREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPA PIEKTISKAKGQP REPQVYTLPPSRDELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTP PVLDSDGSFFL YSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPG K
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83917 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution. pI: ab83917 separates into a number of glycoforms with an observed pI between 5.5 and 9.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified CD40- Fc Chimera that has a predicted pI of 6.0.
    WB WB: Use at an assay dependent dilution. Ab83917 can be used as a WB positive control in conjunction with ab13545. Molecular Mass: Under reducing conditions ab83917 migrates as a broad band between 50 and 65 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified CD40-Fc Chimera that has a predicted monomeric molecular mass of 46.5 kDa.
    Functional Studies FuncS: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        AI326936B cell associated molecule CD40B cell surface antigen CD40
        B cell-associated moleculeB-cell surface antigen CD40Bp50CD 40CD40CD40CD40 antigenCD40 antigen (TNF receptor superfamily member 5)CD40 moleculeCD40 molecule, TNF receptor superfamily member 5CD40 proteinCD40 type II isoformCD40L receptorCDw40GP39HIGM1IGMIMD3MGC9013Nerve growth factor receptor related B lymphocyte activation moleculeOTTHUMP00000031699OTTHUMP00000031700p50T-BAMTBAMTNF receptor superfamily member 5TNFRSF5TNR5_HUMANTRAPTumor necrosis factor receptor superfamily , member 5Tumor necrosis factor receptor superfamily member 5Tumor necrosis factor receptor superfamily member 5 precursorTumor necrosis factor receptor superfamily, member 5, isoform CRA_a
      see all
  • FunctionReceptor for TNFSF5/CD40LG.
  • Tissue specificityB-cells and in primary carcinomas.
  • Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession P25942 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    CD40 protein (Active) images

    • Functional Studies - CD40 protein (Fc Chimera Active) (ab83917)
      Functional Studies - CD40 protein (Fc Chimera Active) (ab83917)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MW and pI of the protein.
    • SDS-PAGE - CD40 protein (Fc Chimera Active) (ab83917)
      SDS-PAGE - CD40 protein (Fc Chimera Active) (ab83917)
      1D SDS-PAGE of ab83917 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab83917
      Lane 2: ab83917 treated with PNGase F to remove potential N-linked glycans
      Lane 3: ab83917 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
      Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie.
    • Western blot
      Western blot
      Anti-CD40 antibody (ab13545) at 1/1000 dilution + CD40 protein (Active) (ab83917) at 1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 8 minutes

    References for CD40 protein (Active) (ab83917)

    ab83917 has not yet been referenced specifically in any publications.

    Publishing research using ab83917 ? Please let us know so that we can cite the reference in this datasheet

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