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CD40L Mouse ELISA Kit (with color giving dyes) (ab119517)

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Overview

  • Product nameCD40L Mouse ELISA Kit (with color giving dyes)See all CD40L kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 6.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 11.1%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich
  • Sensitivity
    0.14 ng/ml
  • Range
    0.31 ng/ml - 20 ng/ml
  • Recovery

    85 %

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab119517 CD40L Mouse ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of mouse sCD40L. ab119517 is for research use only and is not for diagnostic or therapeutic procedures.

    The murine cell surface antigen is known also as Ly62. CD40 belongs to the TNF receptor superfamily and is a biochemical marker for the identification of activated platelets of patients with cardiovascular diseases (angina pectoris, infarction, stroke etc.)

    A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
  • Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications    The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession P29965 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CD154CD40 antigen ligandCD40 ligand
        CD40-LCD40L_HUMANCD40LGgp39HIGM1IGMIMD3soluble formT B cell activating moleculeT BAMT cell antigen Gp39 T-cell antigen Gp39TNF related activation proteinTNF-related activation proteinTNFSF5TrAPTumor necrosis factor (ligand) superfamily member 5Tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)Tumor necrosis factor ligand superfamily member 5
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119517 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      CD40L Mouse ELISA Kit (with color giving dyes) images

      • Sandwich ELISA - CD40L Mouse ELISA Kit (with color giving dyes) (ab119517)
        Sandwich ELISA - CD40L Mouse ELISA Kit (with color giving dyes) (ab119517)
        Representative standard curve using ab119517.

      Protocols

      References for CD40L Mouse ELISA Kit (with color giving dyes) (ab119517)

      ab119517 has not yet been referenced specifically in any publications.

      Publishing research using ab119517 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"