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Overview

  • Product nameCD40L protein (His tag)See all CD40L proteins and peptides ...
  • Protein descriptionRecombinant full length Human soluble CD40L (amino acids 113-261) with an N terminal hexahistidine tag; 149 amino acids without tag, Predicted MWt 17 kDa (UniProt P29965). .
  • Uniprot accessionP29965
  • Molecular weight17kDa
  • Protein length149 amino acids
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferpH: 8.00
    Constituents: 50% Glycerol, 0.32% Tris HCl
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab119150 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution.
    ELISA ELISA: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent concentration.
    Dot Blot Dot: Use at an assay dependent concentration.

    Protein info

    • Alternative names
        CD 40LCD154CD40 antigen ligand
        CD40 ligandCD40 ligand, soluble formCD40-LCD40L_HUMANCD40LGgp39hCD40LHIGM1IGMIMD3T B cell activating moleculeT BAMT-cell antigen Gp39TNF-related activation proteinTNFSF5TrAPTumor necrosis factor (ligand) superfamily member 5Tumor necrosis factor ligand superfamily member 5
      see all
  • FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
  • Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications    The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession P29965 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    CD40L protein (His tag) images

    • SDS-PAGE - CD40L protein (His tag) (ab119150)
      SDS-PAGE - CD40L protein (His tag) (ab119150)
      SDS-PAGE analysis of ab119150.

    References for CD40L protein (His tag) (ab119150)

    ab119150 has not yet been referenced specifically in any publications.

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