Anti-CD43 antibody [MEM-59] (ab9088)
Key features and details
- Mouse monoclonal [MEM-59] to CD43
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-CD43 antibody [MEM-59]
See all CD43 primary antibodies -
Description
Mouse monoclonal [MEM-59] to CD43 -
Host species
Mouse -
Specificity
Recognizes neuraminidase-sensitive epitope on human CD43 molecule (Leukosialin), which is involved in lymphocyte activation. -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD43. Human T lymphocytes
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Positive control
- IHC-P: Human spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-59 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
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IHC-P |
Use a concentration of 10 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 1 - 5 µg/ml. |
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
Tissue specificity
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
Post-translational
modificationsGlycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6693 Human
- Omim: 182160 Human
- SwissProt: P16150 Human
- Unigene: 632188 Human
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Alternative names
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
Images
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Flow cytometric analysis of Human Peripheral Blood cells labelling CD43 with ab9088 at 2 ug/ml showing separation of human CD43 positive lymphocytes (red-filled) from human CD43 negative blood debris (black-dashed).
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Immunohistochemical analysis of Paraffin-embedded sections Human Spleen tissue labelling CD43 with ab9088.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab9088 has been referenced in 7 publications.
- Martin-Jaular L et al. Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO J 40:e105492 (2021). PubMed: 33709510
- Wisnovsky S et al. Genome-wide CRISPR screens reveal a specific ligand for the glycan-binding immune checkpoint receptor Siglec-7. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33495350
- Cermak L et al. Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem 277:7955-61 (2002). PubMed: 11773067
- Alvarado M et al. MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol 25:1051-5 (1995). PubMed: 7537669
- Deckert M et al. CD59 molecule: a second ligand for CD2 in T cell adhesion. Eur J Immunol 22:2943-7 (1992). PubMed: 1385156
- Stefanova I et al. Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol 26:153-61 (1989). PubMed: 2918859
- Stefanova I et al. Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. Folia Biol (Praha) 34:255-65 (1988). PubMed: 2467832