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Overview

  • Product nameCD44 Human ELISA KitSee all CD44 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 9.85ng/ml 0.155 1.52%
    Sample B 8 10.5ng/ml 0.055 4.76%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 50 9.95ng/ml 0.307 3.08%
    Sample B 50 10.7ng/ml 0.105 9.34%
  • Tests
    2 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 0.13 ng/ml
  • Range
    0.31 ng/ml - 10 ng/ml
  • Assay time
    2h 10m
  • Species reactivity
    Reacts with: Human
  • Product overview

    INTENDED USE

    The Human CD44 ELISA is to be used for the in-vitro quantitative determination of soluble CD44 (CD44/phagocytic glycoprotein-1 Pgp-1) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD44. This kit has been configured for research use only and is not to be used in diagnostic procedures.

    PRINCIPLE OF THE METHOD

    The CD44 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for CD44 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD44 concentrations and unknowns are pipetted into these wells.

    During the first incubation, the CD44 antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for CD44 is incubated. Then the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which acts on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD44 present in the samples.

     

    Recognizes both natural and recombinant human CD44

    Range: 0.31 ng/ml - 10 ng/ml

    Sensitivity <0.13 ng/ml

  • Notes

    Safety Information:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceCD44 is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases. CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
  • Cellular localizationCell Membrane; single-pass type I membrane protein.
    • Alternative names
        CD44 antigenCD44 antigen (homing function and Indian blood group system)CD44 molecule
        CD44 molecule (Indian blood group)CD44RCDW44 CDW44 antigenECMR IIIECMRIII EpicanExtracellular matrix receptor IIIGP90 lymphocyte homing/adhesion receptorHCELLHeparan sulfate proteoglycanHermes antigenHUTCH IHUTCHIHyaluronate receptorINLHRMC56MDU2MDU3MGC10468MIC4MUTCH IMUTCHIPGP 1PGP1Phagocytic glycoprotein I
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab45913 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA
    • Application notesELISA

      • CD44 Human ELISA Kit images

      • ELISA - Human CD44 ELISA kit (ab45913)
        ELISA - Human CD44 ELISA kit (ab45913)
        Typical CD44 standard curve ranging from 10 to 0.3 ng/mL

      References for CD44 Human ELISA Kit (ab45913)

      ab45913 has not yet been referenced specifically in any publications.

      Publishing research using ab45913 ? Please let us know so that we can cite the reference in this datasheet

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              I have just purchased an ELISA kit (#ab45913) to use for testing urine. Should I use the standard diluent or PBS to dilute the samples?    

      Thank you for contacting us. The standard diluent will be appopriate for urine samples and is the better choice, compared to PBS. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"