CD44 Human ELISA Kit (ab45912)
- Product nameCD44 Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Sample A 8 9.85ng/ml 1.55 1.52% Sample B 8 10.5ng/ml 0.055 4.76% Inter-assay Sample n Mean SD CV% Sample A 50 9.95ng/ml 0.307 3.08% Sample B 50 1.07ng/ml 0.105 9.34%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity< 0.13 ng/ml
- Range0.31 ng/ml - 10 ng/ml
- Assay time2h 10m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s CD44 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble CD44 in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for CD44 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD44 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD44 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD44 present in the samples.
This kit will recognize both endogenous and recombinant Human CD44.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-CD44 anitibody Red 1 x 0.3ml 2 x 0.3ml CD44 Control Silver 2 vials 4 vials CD44 Microplate (12 x 8 well strips) 1 unit 2 units CD44 Standard Yellow 2 vials 4 vials Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl
- Research Areas
- RelevanceCD44 is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases. CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
- Cellular localizationCell Membrane; single-pass type I membrane protein.
- CD44 antigen
- CD44 antigen (homing function and Indian blood group system)
- CD44 molecule
- CD44 molecule (Indian blood group)
- CDW44 antigen
- ECMR III
- Extracellular matrix receptor III
- GP90 lymphocyte homing/adhesion receptor
- Heparan sulfate proteoglycan
- Hermes antigen
- HUTCH I
- Hyaluronate receptor
- MUTCH I
- PGP 1
- Phagocytic glycoprotein I
Our Abpromise guarantee covers the use of ab45912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for CD44 Human ELISA Kit (ab45912)
This product has been referenced in:
- Simpson KL et al. A caspase-3 'death-switch' in colorectal cancer cells for induced and synchronous tumor apoptosis in vitro and in vivo facilitates the development of minimally invasive cell death biomarkers. Cell Death Dis 4:e613 (2013). ELISA ; Human . Read more (PubMed: 23640455) »
- Urquidi V et al. CCL18 in a Multiplex Urine-Based Assay for the Detection of Bladder Cancer. PLoS One 7:e37797 (2012). ELISA ; Human . Read more (PubMed: 22629457) »