Overview

  • Product nameCD44 Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Inter-assay
    Sample n Mean SD CV%
    Sample A 50 9.95ng/ml 0.307 3.08%
    Sample B 50 1.07ng/ml 0.105 9.34%
    Sample A 50 9.95ng/ml 0.307 3.08%
    Sample B 50 1.07ng/ml 0.105 9.34%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.13 ng/ml
  • Range
    0.31 ng/ml - 10 ng/ml
  • Assay time
    2h 10m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD44 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble CD44 in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for CD44 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD44 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD44 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD44 present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD44.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceCD44 is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases. CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
  • Cellular localizationCell Membrane; single-pass type I membrane protein.
  • Alternative names
    • CD44 antigen
    • CD44 antigen (homing function and Indian blood group system)
    • CD44 molecule
    • CD44 molecule (Indian blood group)
    • CD44R
    • CDW44
    • CDW44 antigen
    • ECMR III
    • ECMRIII
    • Epican
    • Extracellular matrix receptor III
    • GP90 lymphocyte homing/adhesion receptor
    • HCELL
    • Heparan sulfate proteoglycan
    • Hermes antigen
    • HUTCH I
    • HUTCHI
    • Hyaluronate receptor
    • IN
    • LHR
    • MC56
    • MDU2
    • MDU3
    • MGC10468
    • MIC4
    • MUTCH I
    • MUTCHI
    • PGP 1
    • PGP1
    • Phagocytic glycoprotein I
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab45912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

CD44 Human ELISA Kit images

  • Representative Standard Curve using ab45912

Protocols

References for CD44 Human ELISA Kit (ab45912)

This product has been referenced in:
  • Simpson KL  et al. A caspase-3 'death-switch' in colorectal cancer cells for induced and synchronous tumor apoptosis in vitro and in vivo facilitates the development of minimally invasive cell death biomarkers. Cell Death Dis 4:e613 (2013). ELISA ; Human . Read more (PubMed: 23640455) »
  • Urquidi V  et al. CCL18 in a Multiplex Urine-Based Assay for the Detection of Bladder Cancer. PLoS One 7:e37797 (2012). ELISA ; Human . Read more (PubMed: 22629457) »

See all 3 Publications for this product

Product Wall

Thank you for your interest in the CD44 and CD56 ELISA kits, ab45912 and ab119587. Neither has been tested for reactivity with mouse or rat samples. We are unable to assess the probability of cross-reaction by BLAST, as the locations of the epitopes th...

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Thank you for contacting Abcam regarding ab45912.


The wash buffer we recommend if you have run out is PBS with 0.05% Tween 20.


I hope this information is helpful. Please do not hesitate to contact us if you have any addition...

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Thank you for the question and for the customer to have taken the time to illustrate the question.

The laboratory confirmed thatboth antibodies will recognize the part after the cleavage site - the part which will be detached from the cells -...

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Thank you for your inquiry and your patience.

Unfortunately the antibody used as detection antibody has not been tested in any other application as IHC or ICC. We do therefore not know whether it can detect the CD44 on the cells.

Th...

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Thank you for your enquiry and your interest. This product has been specifically tested and it is suitable for cell culture supernatant, serum, plasma. We have not tested in other body fluids. For further information, please take a look ...

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Thank you for your enquiry about ab45912. The constitutions of recommended lysis buffer is as follows; NaCl 150mM, NP-40 1%, Tris-HCl 20mM -adjust to pH=8 with protease inhibitors. I hope this information helps you, and please do not hesitate to con...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"