You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameCD44 Human ELISA KitSee all CD44 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 9.85ng/ml 1.55 1.52%
    Sample B 8 10.5ng/ml 0.055 4.76%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 50 9.95ng/ml 0.307 3.08%
    Sample B 50 1.07ng/ml 0.105 9.34%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 0.13 ng/ml
  • Range
    0.31 ng/ml - 10 ng/ml
  • Assay time
    2h 10m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD44 Human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human serum, plasma, buffered solutions and cell culture media.

     

    Principal

    The CD44 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for CD44 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD44 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD44 antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for CD44 is incubated. Then the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which actes on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD44 present in the samples.

     

    Recognizes both natural and recombinant Human CD44 Range: 0.31 ng/ml - 10 ng/ml Sensitivity

  • Notes

    Safety Information:

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

     

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

     

     

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

     

     

    • Do not pipette by mouth.

     

     

    • For research use only.

     

     

    • The stop solution in this kit contains 9.8% sulphuric acid.

     

  • Tested applicationsELISA more details

Properties

  • RelevanceCD44 is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases. CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
  • Cellular localizationCell Membrane; single-pass type I membrane protein.
    • Alternative names
        CD44 antigenCD44 antigen (homing function and Indian blood group system)CD44 molecule
        CD44 molecule (Indian blood group)CD44RCDW44 CDW44 antigenECMR IIIECMRIII EpicanExtracellular matrix receptor IIIGP90 lymphocyte homing/adhesion receptorHCELLHeparan sulfate proteoglycanHermes antigenHUTCH IHUTCHIHyaluronate receptorINLHRMC56MDU2MDU3MGC10468MIC4MUTCH IMUTCHIPGP 1PGP1Phagocytic glycoprotein I
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab45912 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      CD44 Human ELISA Kit images

      • ELISA - Human CD44 ELISA Kit (ab45912)
        ELISA - Human CD44 ELISA Kit (ab45912)
        Typical CD44 standard curve.

      References for CD44 Human ELISA Kit (ab45912)

      This product has been referenced in:
      • Urquidi V  et al. CCL18 in a Multiplex Urine-Based Assay for the Detection of Bladder Cancer. PLoS One 7:e37797 (2012). ELISA ; Human . Read more (PubMed: 22629457) »
      • Chen R  et al. Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions. PLoS Comput Biol 6: (2010). ELISA ; Human . Read more (PubMed: 20885780) »

      See all 2 Publications for this product

      Publishing research using ab45912 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab45912 Submit a Question for ab45912

      Displaying 1 - 6 of 6 results for Abreviews and Q&A

      10
      Votes: Highest First

      can you tell me if this kit has ever been tested in mouse or rat, and if not, if you could BLAST the immunogen sequences? I am looking for a kit that will not cross-react...

      Thank you for your interest in the CD44 and CD56 ELISA kits, ab45912 and ab119587. Neither has been tested for reactivity with mouse or rat samples. We are unable to assess the probability of cross-reaction by BLAST, as the locations of the epitopes that ...

      Read More

      Is for my elisa kit : The ab45912 kit wash buffer PBS or TBS?

      Thank you for contacting Abcam regarding ab45912.


      The wash buffer we recommend if you have run out is PBS with 0.05% Tween 20.


      I hope this information is helpful. Please do not hesitate to contact us if you have any additional ...

      Read More

      Dear Technical support team,

      Pls attached this file.

      And the customer want to know that where is the epitope that the antibodydetected?

      Thank you very much.

      Best regards,

      Thank you for the question and for the customer to have taken the time to illustrate the question.

      The laboratory confirmed thatboth antibodies will recognize the part after the cleavage site - the part which will be detached from the cells - pl...

      Read More

      Dear Technical support teams,

      Could you pls check the cat.no. of antibody that use in the ab45912 >>> CD44 Human ELISA Kit (ab45912) ?

      Because the customer want to detect the CD44with 2 system. (soluble form and on cell surf...

      Read More

      Thank you for your inquiry and your patience.

      Unfortunately the antibody used as detection antibody has not been tested in any other application as IHC or ICC. We do therefore not know whether it can detect the CD44 on the cells.

      The c...

      Read More

      i have a question regarding to Human CD44 Elisa Kit - 1 x 96 Well Plate. could you tell me if the test is effective for detection of cd44 in saliva or oral rinses?

      Thank you for your enquiry and your interest. This product has been specifically tested and it is suitable for cell culture supernatant, serum, plasma. We have not tested in other body fluids. For further information, please take a look at ...

      Read More

      I have a question regarding to Human CD44 ELISA Kit - 1 x 96 Well Plate, ab45912. Could you tell me the suitable lysis buffer for solubilising plasma membrane to detect CD44 by this Kit?

      Thank you for your enquiry about ab45912. The constitutions of recommended lysis buffer is as follows; NaCl 150mM, NP-40 1%, Tris-HCl 20mM -adjust to pH=8 with protease inhibitors. I hope this information helps you, and please do not hesitate to contac...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"