![Western blot - CD44 antibody [EPR1013Y] (ab51037) image](#ab51037_1.bmp)
![Immunohistochemistry (Paraffin-embedded sections) - CD44 antibody [EPR1013Y] (ab51037) image](#ab51037_2.bmp)
![Flow Cytometry - Anti-CD44 antibody [EPR1013Y] (ab51037) image](#CD44-Primary-antibodies-ab51037-15.jpg)
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Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-CD44 antibody [EPR1013Y]
See all CD44 products (79) ...
Description
Rabbit monoclonal [EPR1013Y] to CD44
Specificity
We received negative feedback on the reactivity of this antibody in mouse and rat species, and for mouse and rat this antibody is recommended for WB only.
Tested applications
IHC-P, IHC-Fr, ICC/IF, WB, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: TRYVQKGEYRTNPEDIYPS, corresponding to amino acids 153-171 of Human CD44
TRYVQKGEYR TNPEDIYPS
Positive control
TF-1 cell lysate or human urinary bladder tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR1013Y
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
ICC/IF
ICC/IF: 1/100 - 1/250.ICC/IF: 1/100 - 1/250.
WB: 1/5000Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
Flow Cyt: 1/30
Application notes
Is unsuitable for or IP.
Target
Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similarities
Contains 1 Link domain.
Domain
The lectin-like LINK domain is responsible for hyaluronan binding.
Post-translational
modifications
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
Cellular localization
Membrane.
Target information above from: UniProt accessionP16070
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Database links
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
Anti-CD44 antibody [EPR1013Y] images:
Western blot - CD44 antibody [EPR1013Y] (ab51037)
![Western blot - CD44 antibody [EPR1013Y] (ab51037)](/ps/datasheet/Images/51/ab51037/ab51037_1.bmp)
Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/5000 dilution + TF-1 cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled. at 1/2000 dilution
Predicted band size : 82 kDa
Observed band size : 82 kDa
Immunohistochemistry (Paraffin-embedded sections) - CD44 antibody [EPR1013Y] (ab51037)
![Immunohistochemistry (Paraffin-embedded sections) - CD44 antibody [EPR1013Y] (ab51037)](/ps/datasheet/Images/51/ab51037/ab51037_2.bmp)
Ab51037 (1:100) staining human CD44 in human urinary bladder tissue by immunohistochemistry using paraffin embedded tissue.
Flow Cytometry - Anti-CD44 antibody [EPR1013Y] (ab51037)
![Flow Cytometry - Anti-CD44 antibody [EPR1013Y] (ab51037)](/ps/datasheet/images/51/ab51037/CD44-Primary-antibodies-ab51037-15.jpg)
ab51037 used at a 1/30 dilution to detect CD44 in human MDA-MB-231 cells by Flow Cytometry. The secondary used was a Phycoerythrin conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.The purple plot indicates IgG isotype control and the green plot represents CD44.
Image courtesy of an anonymous Abreview.
References for Anti-CD44 antibody [EPR1013Y] (ab51037)
This product has been referenced in:
- Rybak APet al. Characterization of sphere-propagating cells with stem-like properties from DU145 prostate cancer cells. Biochim Biophys Acta 1813:683-94 (2011). IHC-P; Human.Read more (PubMed: 21277911) »
- Kai Met al. Semi-quantitative evaluation of CD44(+) /CD24(-) tumor cell distribution in breast cancer tissue using a newly developed fluorescence immunohistochemical staining method. Cancer Sci : (2011).Read more (PubMed: 21838786) »
See all 8 publications for this product
Publishing research using ab51037? Please let us know so that we can cite the reference in this datasheet
![CD44 antibody [EPR1013Y] for WB in Human (51037)](/ps/datasheet/images/51/ab51037/CD44-Primary-antibodies-ab51037-2.jpg)
![Anti-CD44 antibody [EPR1013Y] for Flow Cytometry in Human (51037)](/ps/datasheet/images/51/ab51037/CD44-Primary-antibodies-ab51037-13.JPG)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"