CD44 antibody [EPR1013Y] (ab51037)

Product Name 

CD44 antibody [EPR1013Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EPR1013Y] to CD44

Immunogen 

Synthetic peptide: TRYVQKGEYRTNPEDIYPS, corresponding to amino acids 153-171 of Human CD44
BLAST 'TRYVQKGEYRTNPEDIYPS' with or

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/30.
ICC/IF: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

WB: 1/5000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).

Is unsuitable for IP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

TF-1 cell lysate or human urinary bladder tissue.

Cellular localization 

Type I membrane protein.

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Adhesion

Relevance 

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EPR1013Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for CD44 antibody [EPR1013Y] images, references, products related to ab51037 and other tools.

CD44 antibody [EPR1013Y] images:

References for CD44 antibody [EPR1013Y] (ab51037)

  This product has been used in:

Chen H et al. Coexpression of invasive markers (uPA, CD44) and multiple drug-resistance proteins (MDR1, MRP2) is correlated with epithelial ovarian cancer progression. Br J Cancer 101:432-40 (2009). ICC/IF, IHC-P; Human. PubMed: 19603017

If you publish research using ab51037 please let us know so that we can cite the reference on this datasheet.

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