![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD44 antibody [F10-44-2] (ab6124) image](#CD44-Primary-antibodies-ab6124-5.jpg)
![Flow Cytometry - CD44 antibody [F10-44-2] (ab6124) image](#CD44-Primary-antibodies-ab6124-6.jpg)
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Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-CD44 antibody [F10-44-2]
See all CD44 products (79) ...
Description
Mouse monoclonal [F10-44-2] to CD44
Tested applications
IHC-Fr, IP, Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
CD44-positive cell preparation.
Positive control
In Flow Cytometry, this antibody gave a positive signal in peripheral blood lymphocytes. In IHC, this antibody gave a positive signal in human kidney carcinoma sections.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
F10-44-2
Isotype
IgG2a
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use a concentration of 0.5 - 2 µg/ml.
IP: Use a concentration of 0.5 - 2 µg/ml.
Flow Cyt: Use 0.5-1µg for 106 cells.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similarities
Contains 1 Link domain.
Domain
The lectin-like LINK domain is responsible for hyaluronan binding.
Post-translational
modifications
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
Cellular localization
Membrane.
Target information above from: UniProt accessionP16070
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Database links
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
Anti-CD44 antibody [F10-44-2] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD44 antibody [F10-44-2] (ab6124)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD44 antibody [F10-44-2] (ab6124)](/ps/datasheet/images/6/ab6124/CD44-Primary-antibodies-ab6124-5.jpg)
IHC image of CD44 staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6124, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow Cytometry - CD44 antibody [F10-44-2] (ab6124)
![Flow Cytometry - CD44 antibody [F10-44-2] (ab6124)](/ps/datasheet/images/6/ab6124/CD44-Primary-antibodies-ab6124-6.jpg)
Overlay histogram showing peripheral blood lymphocytes stained with ab6124 (red line). The cells were incubated with the antibody (ab6124, 0.5µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
References for Anti-CD44 antibody [F10-44-2] (ab6124)
This product has been referenced in:
- Le Bras GFet al. CD44 Upregulation in E-Cadherin-Negative Esophageal Cancers Results in Cell Invasion. PLoS One 6:e27063 (2011). IHC-P; Human.Read more (PubMed: 22069487) »
- Hunt ME & Brown DR Role of sialidase in Mycoplasma alligatoris-induced pulmonary fibroblast apoptosis. Vet Microbiol 121:73-82 (2007).Read more (PubMed: 17276629) »
See all 3 publications for this product
Publishing research using ab6124? Please let us know so that we can cite the reference in this datasheet
![CD44 antibody [F10-44-2] for IHC-P in Human (6124)](/ps/Reviews/Images/ab6181_52661.jpg)
![CD44 antibody [F10-44-2] for IHC-P in Human (6124)](/ps/Reviews/Images/ab13858_82452.jpg)
![CD44 antibody [F10-44-2] for IHC-P in Human (6124)](/ps/datasheet/images/6/ab6124/CD44-Primary-antibodies-ab6124-2.JPG)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"