Anti-CD44 antibody [IM7] (FITC) (ab19622)

Immunocytochemistry/ Immunofluorescence - CD44 antibody [IM7] (FITC) (ab19622)

ab19622 at 1/400 staining human prostate cells by ICC/IF.  The cells were paraformaldehyde fixed and blocked with 20% serum for 1 h at 24°C before incubation with the primary antibody for 20 hours at 4°C. An Alexa Fluor® conjugated donkey anti-rat was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Ville Harma

Flow Cytometry - CD44 antibody [IM7] (FITC) (ab19622)

Overlay histogram showing MCF-7 cells stained with ab19622 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19622, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2µg/1x10⁶ cells) with secondary antibody DyLight® 488 goat anti-rat IgG (Fc) (ab96971) at 1/250 dilution for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in non-fixed and methanol (5 min) fixed MCF-7 cells used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [IM7] (FITC) (ab19622)

ab19622 staining CD44 in Pig skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer (10mM; pH6.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. An undiluted Biotin-conjugated Donkey anti-rat IgG polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview