CD44 antibody [IM7] (FITC) (ab19622)

Product Name 

CD44 antibody [IM7] (FITC)

Product type 

Primary antibodies

Description 

Rat monoclonal [IM7] to CD44 (FITC)

Immunogen 

Unfortunately, this information is considered to be commercially sensitive

Reacts with 
(species key)

Hu, Cat, Cow, Dog, Hrs, Pig

Specificity 

Recognizes all isoforms of the Pgp-1 cell surface glycoprotein. Present on B-cells, monocytes, macrophages and certain subsets of thymocytes and peripheral T-cells. Mice with the Ly-24.1 allotype have high densities of CD44+ T-cells, while Ly-24.2 strains are low expressors.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-Fr, IP

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use 1µg for 10⁶ cells.
IHC-Fr (acetone fixed): Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Type I membrane protein.

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Adhesion

Relevance 

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

IM7

Isotype 

IgG2b

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 1.0% BSA, 0.15M Sodium chloride, 0.01M Potassium phosphate. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

See below for CD44 antibody [IM7] (FITC) images, references, products related to ab19622 and other tools.

CD44 antibody [IM7] (FITC) images:

References for CD44 antibody [IM7] (FITC) (ab19622)

  This product has been used in: (two most recent references)

Bilgen B et al. CD14-negative isolation enhances chondrogenesis in synovial fibroblasts. Tissue Eng Part A 15:3261-70 (2009). Flow Cyt; Pig. PubMed: 19382853

Hunt ME & Brown DR Role of sialidase in Mycoplasma alligatoris-induced pulmonary fibroblast apoptosis. Vet Microbiol 121:73-82 (2007). PubMed: 17276629

See all 3 publication references for this product.


If you publish research using ab19622 please let us know so that we can cite the reference on this datasheet.

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