Anti-CD44 antibody [KM201] (ab25340)

Overview

• Product nameAnti-CD44 antibody [KM201]See all CD44 primary antibodies ...
• Description
  Rat monoclonal [KM201] to CD44
• Tested applicationsIP, WB, Flow Cyt, IHC-FoFr, IHC-P more details
• Species reactivity
  Reacts with: Mouse
  
• Immunogen

  The details of the immunogen for this antibody are not available.

• Epitopeab25340 reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule.
• General notes

  
  

  ab25340 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and WhitlockWitte Cultures, prevent the earliest intrathymic precursors from homing to the thymus and costimulate the activation of freshly purified splenic CD4+ T cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 100mM Borate buffered saline, pH 8.2
• Concentration information loading...
• PurityIgG fraction
• Primary antibody notes ab25340 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and WhitlockWitte Cultures, prevent the earliest intrathymic precursors from homing to the thymus and costimulate the activation of freshly purified splenic CD4+ T cells.
• Clonality Monoclonal
• Clone numberKM201
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Neutrophil Lineage

  • Stem Cells
  • Mesenchymal Stem Cells
  • Surface Molecules

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • T Lymphocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Dendritic Cell Lineage

  • Cancer
  • Tumor immunology
  • CD markers

  • Cancer
  • Tumor biomarkers
  • Other

  • Immunology
  • Cell Type Markers
  • CD
  • Adhesion

Applications

Target

• FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
• Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
• Sequence similaritiesContains 1 Link domain.
• DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
• Post-translational
  modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
  N-glycosylated.
  O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
  Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
• Cellular localizationMembrane.

Target information above from: UniProt accession P16070 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 12505 Mouse
  • SwissProt: P15379 Mouse
  • Unigene: 423621 Mouse

• Alternative names
  LHR antibodyBA-1 antibodyCD 44 antibody

  CD44 antibodyCD44 antibodyCD44 antigen antibodyCD44 isoform RC antibodyCD44 molecule antibodyCD44_HUMAN antibodyCDw44 antibodyCell surface glycoprotein CD44 antibodychondroitin sulfate proteoglycan 8 antibodyECM III antibodyECMR III antibodyECMR-III antibodyECMRIII antibodyEpican antibodyExtracellular Matrix Receptor III antibodyGP90 Lymphocyte Homing Adhesion Receptor antibodyGP90 lymphocyte homing/adhesion receptor antibodyHCAM antibodyHCELL antibodyhematopoietic cell E- and L-selectin ligand antibodyHeparan Sulfate Proteoglycan antibodyHermes Antigen antibodyhoming function and Indian blood group system antibodyHSA antibodyHUTCH I antibodyHUTCH-I antibodyHUTCH1 antibodyHyaluronate Receptor antibodyIN antibodyIndian blood group antibodyInlu Related p80 Glycoprotein antibodylhr antibodyLy 24 antibodyMC56 antibodyMDU2 antibodyMDU3 antibodyMGC10468 antibodyMIC4 antibodyMUTCH I antibodyMUTCH1 antibodyPGP 1 antibodyPGP-1 antibodyPGP-I antibodyPGP1 antibodyPhagocytic Glycoprotein 1 antibodyPhagocytic glycoprotein I antibodyTransmembrane glycoprotein antibody

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Anti-CD44 antibody [KM201] images

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  Blocking - Anti-CD44 antibody [KM201] (ab25340)Image from Goncharova V et al., J Biol Chem. 2012 Jul 20;287(30):25419-33. Epub 2012 May 31.; Fig S3.; doi: 10.1074/jbc.M112.376699; July 20, 2012 The Journal of Biological Chemistry, 287, 25419-25433.
  Effect of CD44 blockade on hematopoiesis.
  Long term bone marrow cultures from Has1/2/3 triple knock-out mice were treated with 300 µM 4MU or vehicle control during weekly feedings. Where indicated, 100 µg/ml HMW HA (UC, 600-1,500 kD) or 10 µg/ml CD44-specific antibody (ab25340) were added.