Overview

  • Product nameAnti-CD44 antibody [KM201]See all CD44 primary antibodies ...
  • Description
    Rat monoclonal [KM201] to CD44
  • Tested applicationsIP, WB, Flow Cyt, IHC-FoFr, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Epitopeab25340 reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule.
  • General notes


    ab25340 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and WhitlockWitte Cultures, prevent the earliest intrathymic precursors from homing to the thymus and costimulate the activation of freshly purified splenic CD4+ T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab25340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
Flow Cyt
IHC-FoFr
IHC-P
IHC-Fr
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    IHC-FoFr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 85 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
    • Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
    • Sequence similaritiesContains 1 Link domain.
    • DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
    • Post-translational
      modifications
      Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
      N-glycosylated.
      O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
      Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 isoform RC antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECM III antibody
      • ECMR III antibody
      • ECMR-III antibody
      • ECMRIII antibody
      • Epican antibody
      • Extracellular Matrix Receptor III antibody
      • GP90 Lymphocyte Homing Adhesion Receptor antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCAM antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan Sulfate Proteoglycan antibody
      • Hermes Antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH I antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • Hyaluronate Receptor antibody
      • IN antibody
      • Indian blood group antibody
      • Inlu Related p80 Glycoprotein antibody
      • lhr antibody
      • Ly 24 antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH I antibody
      • MUTCH1 antibody
      • PGP 1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic Glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      • Transmembrane glycoprotein antibody
      see all

    Anti-CD44 antibody [KM201] images

    • Effect of CD44 blockade on hematopoiesis.
      Long term bone marrow cultures from Has1/2/3 triple knock-out mice were treated with 300 µM 4MU or vehicle control during weekly feedings. Where indicated, 100 µg/ml HMW HA (UC, 600-1,500 kD) or 10 µg/ml CD44-specific antibody (ab25340) were added.

    References for Anti-CD44 antibody [KM201] (ab25340)

    This product has been referenced in:
    • Goncharova V  et al. Hyaluronan expressed by the hematopoietic microenvironment is required for bone marrow hematopoiesis. J Biol Chem 287:25419-33 (2012). Blocking ; Mouse . Read more (PubMed: 22654110) »
    • Gazi U  et al. Fungal Recognition Enhances Mannose Receptor Shedding through Dectin-1 Engagement. J Biol Chem 286:7822-9 (2011). WB ; Mouse . Read more (PubMed: 21205820) »

    See all 8 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample Mouse Tissue sections (spleen)
    Specification spleen
    Permeabilization No
    Fixative Paraformaldehyde
    Username

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    Verified customer

    Submitted Jan 08 2014

    DISCOUNT CODE: *******
    Expiration date: DD MM YYYY

    I am very pleased to hear you would like to accept our offer and test ab25340 in Human and IHC-Fr. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem th...

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    Thank you for contacting us again.

    I am sorry I have over-read your main question. We state only mouse as a tested species on the datasheet so far. All tested applicationsare specified on our datasheets, and these are updated as soon as any ...

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    Thank you for your inquiry.

    Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information. This information is stated on the datasheet in the immunogen section.

    We a...

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    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (T-cell lymphoma)
    Specification T-cell lymphoma
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Blocking step Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 24 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"