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Western blot - CD44 antibody [MEM-263] (ab9524)
![Western blot - CD44 antibody [MEM-263] (ab9524)](/ps/datasheet/images/ab9524_1.jpg)
(enlarge)
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Product Name
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CD44 antibody [MEM-263]
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See all CD44 antibodies (59)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [MEM-263] to CD44
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Immunogen
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COS-7 cells.
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Reacts with
(species key)
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Hu
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Epitope
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extracellular (N-terminal) domain
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Tested applications
(see key)
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Flow Cyt, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at a concentration of 2 µg/ml.
IP: Use at a concentration of 2 µg/ml.
WB: Use at a concentration of 2 µg/ml. Use under non reducing conditions.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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KG1a leukemia acute myeloid cell line
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Cellular localization
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Type I membrane protein.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
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Relevance
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CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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MEM-263
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Isotype
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IgG1
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from ascites by protein-A affinity chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD44 antibody [MEM-263] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD44 antibody [MEM-263] images, references, products related to ab9524 and other tools.
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CD44 antibody [MEM-263] images:
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 Western blot - CD44 antibody [MEM-263] (ab9524)
Western blotting of HPB-ALL cell lysate (non-reduced sample) stained by ab9524.
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Search PubMed (MEDLINE) for references to CD44
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CD44 antibody [MEM-263] - more information
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Customer reviews (feedback) regarding CD44 antibody [MEM-263] |
Customer FAQs regarding CD44 antibody [MEM-263] |
Protocols for CD44 antibody [MEM-263] |
Price and availability of products related to CD44 antibody [MEM-263] |
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CD44 antibody [MEM-263] - related products:
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CD44 antibody [MEM-263] - other tools:
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Contact Abcam with a Technical Enquiry about CD44 antibody [MEM-263] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - CD44 antibody [MEM-263] (ab9524)](/index.html?datasheet=9524#ab9524_1.jpg)
