![Western blot - CD44 antibody [MEM-263] (ab9524) image](#ab9524_1.jpg)
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Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-CD44 antibody [MEM-263]
See all CD44 products (79) ...
Description
Mouse monoclonal [MEM-263] to CD44
Tested applications
IP, WB, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
COS-7 cells.
Epitope
extracellular (N-terminal) domain
Positive control
KG1a leukemia acute myeloid cell line
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascites by protein-A affinity chromatography.
Clonality
Monoclonal
Clone number
MEM-263
Isotype
IgG1
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
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Western blot - CD44 antibody [MEM-263] (ab9524)
![Western blot - CD44 antibody [MEM-263] (ab9524) image](/ps/datasheet/Images/9/ab9524/ab9524_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab9524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use a concentration of 2 µg/ml
WB: Use a concentration of 2 µg/ml Use under non reducing condition.
Flow Cyt: Use a concentration of 2 µg/ml
Target
Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similarities
Contains 1 Link domain.
Domain
The lectin-like LINK domain is responsible for hyaluronan binding.
Post-translational
modifications
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
Cellular localization
Membrane.
Target information above from: UniProt accessionP16070
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Database links
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
Anti-CD44 antibody [MEM-263] images:
Western blot - CD44 antibody [MEM-263] (ab9524)
Western blotting of HPB-ALL cell lysate (non-reduced sample) stained by ab9524.
References for Anti-CD44 antibody [MEM-263] (ab9524)
This product has been referenced in:
- Morigi Met al. Alternative pathway activation of complement by shiga toxin promotes exuberant c3a formation that triggers microvascular thrombosis. J Immunol 187:172-80 (2011). Blocking; Human.Read more (PubMed: 21642543) »
See 1 publication for this product
Publishing research using ab9524? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"