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ab41477 |
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ab41478 has been referenced in 1 publications.
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All lanes : Anti-CD44 antibody (ab41478) at 1 µg/ml
Lane 1 : HUVEC (Human Umbilical vein endothelial cell line) Whole Cell Lysate
Lane 2 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 3 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 30 kDa (possible isoform),35 kDa (possible isoform),70 kDa (possible isoform).
It is probable that this protein runs higher than the predicted MW as it likely to be glycosylated.
2
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