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ab41477 |
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Thank you for checking. I am still puzzled by why abcam states on the data sheetthat the ab reacts with rat if it wasn't quality controlled. |
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ANSWER: |
Yes, it does appear that rat was mistakenly listed as a 'Tested application' and I thank you for bringing this to our attention. Although we have not had a complaint regarding ab41478 in over 3 years, I will remove Rat as a tested species from this product. |
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Is there any rat data available using this antibody? |
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ANSWER: |
I'm afraid we have never tested this on rat – we only test this in house in WB in human lysates and it doesn’t look like there’s any references orAbreviews citing use with rat either. |
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Please suggest a positive control product to use with ab41478 in IHC-P |
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ANSWER: |
Thank you for your telephone enquiry. I can confirm that ab41478 CD44 antibody has been used succesfully on Kidney (human), lung (mouse) and thymus (rat) sections in IHC-P. The details can be found on the Abreview published on the datasheet. (Select the Abreviews tab at the top of the main datasheet page). http://www.abcam.com/index.html?datasheet=41478&tab=abreviews&intabreviewid=10293 I hope this will be helpful to you. if you have any further questions, please do not hesitate to contact us. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-CD44 antibody (ab41478) at 1 µg/ml
Lane 1 : HUVEC (Human Umbilical vein endothelial cell line) Whole Cell Lysate
Lane 2 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 3 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 30 kDa (possible isoform),35 kDa (possible isoform),70 kDa (possible isoform).
It is probable that this protein runs higher than the predicted MW as it likely to be glycosylated.
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