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Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-CD44 antibody
See all CD44 products (79) ...
Description
Rabbit polyclonal to CD44
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human CD44.
(Peptide available as ab41477.)
Positive control
This antibody gave a positive signal in the following human whole cell lysates: HUVEC, HL60 and Y79
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
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Western blot - CD44 antibody (ab41478)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 95 kDa (predicted molecular weight: 82 kDa).
IHC-P: Use at an assay dependent concentration.
Target
Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similarities
Contains 1 Link domain.
Domain
The lectin-like LINK domain is responsible for hyaluronan binding.
Post-translational
modifications
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
Cellular localization
Membrane.
Target information above from: UniProt accessionP16070
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Database links
- Entrez Gene: 960 Human
- Entrez Gene: 12505 Mouse
- Omim: 107269 Human
- SwissProt: P16070 Human
- SwissProt: P15379 Mouse
- Unigene: 502328 Human
- Unigene: 423621 Mouse
Anti-CD44 antibody images:
Western blot - CD44 antibody (ab41478)
All lanes : Anti-CD44 antibody (ab41478) at 1 µg/ml
Lane 1 : HUVEC (Human Umbilical vein endothelial cell line) Whole Cell Lysate
Lane 2 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 3 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 30 kDa (possible isoform),35 kDa (possible isoform),70 kDa (possible isoform).
It is probable that this protein runs higher than the predicted MW as it likely to be glycosylated.
References for Anti-CD44 antibody (ab41478)
This product has been referenced in:
- Bruce KDet al. Maternal high-fat feeding primes steatohepatitis in adult mice offspring, involving mitochondrial dysfunction and altered lipogenesis gene expression. Hepatology 50:1796-808 (2009).Read more (PubMed: 19816994) »
See 1 publication for this product
Publishing research using ab41478? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"