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Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-CD44 (phospho S706) antibody
See all CD44 products (79) ...
Description
Rabbit polyclonal to CD44 (phospho S706)
Specificity
Detects endogenous CD44 only when phosphorylated at serine 706.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphopeptide derived from human CD44 around the phosphorylation site of serine 706.
Positive control
NIH/3T3 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
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Western blot - CD44 (phospho S706) antibody (ab63389)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
WB: 1/500 - 1/1000.Detects a band of approximately 85 kDa (predicted molecular weight: 82 kDa).
Target
Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similarities
Contains 1 Link domain.
Domain
The lectin-like LINK domain is responsible for hyaluronan binding.
Post-translational
modifications
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
Cellular localization
Membrane.
Target information above from: UniProt accessionP16070
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Database links
- Entrez Gene: 960 Human
- Entrez Gene: 12505 Mouse
- Omim: 107269 Human
- SwissProt: P16070 Human
- SwissProt: P15379 Mouse
- Unigene: 502328 Human
- Unigene: 423621 Mouse
Anti-CD44 (phospho S706) antibody images:
Western blot - CD44 (phospho S706) antibody (ab63389)
All lanes : Anti-CD44 (phospho S706) antibody (ab63389) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells,
treated with PMA (250ng/ml, 5mins)
Lane 2 : Extracts from NIH/3T3 cells,
treated with PMA (250ng/ml, 5mins) plus phosphopeptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 82 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
References for Anti-CD44 (phospho S706) antibody (ab63389)
This product has been referenced in:
- Wang YTet al. An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9:5582-97 (2010). WB; Human.Read more (PubMed: 20815410) »
See 1 publication for this product
Publishing research using ab63389? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"