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CD44var (v5) Human ELISA kit (with color giving dyes) (ab119518)

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Overview

  • Product nameCD44var (v5) Human ELISA kit (with color giving dyes)
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5.8%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    0.14 ng/ml
  • Range
    0.31 ng/ml - 10 ng/ml
  • Recovery

    92 %

    Sample specific recovery
    Sample type Average % Range
    Serum 92 84% - 100%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab119518 CD44var (v5) Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD44var (v5). ab119518 is for research use only. Not for diagnostic or therapeutic procedures.

    CD44 is a polymorphic glycoprotein which participates in a wide variety of cell-cell or cell-matrix interactions including lymphocyte homing, establishment of B and T cell immune responses, tumor metastasis formation and inflammation.

    Three isoform categories of the CD44 molecule have been identified:

    1) a predominant 80-90 kDa category, the so-called standard form named CD44std,

    2) an intermediate size category of 110-160 kDa and

    3) a category which includes very large isoforms of 250 kDa covalently modified by the addition of chondroitin sulfate.

    This CD44-family of transmembrane receptor molecules is derived from a single gene located on chromosome 11. Alternative splicing of the mRNA gives rise to the different isoforms, containing inserts of varying sizes in the extracellular domain of the molecule (exons v2-v10). In contrast to standard CD44 (CD44std) which is almost ubiquitously expressed, the variety of CD44 isoforms (CD44var) have a much more restricted distribution, e.g., on keratinocytes (exons v3-v10), epithelial cells (exons v8-v10), activated lymphocytes and macrophages (exon v6).

    A splice variant of CD44 (exons v4-v7) confers metastatic behavior in a rat carcinoma model; aberrant expression of splice variants has been detected on a variety of human tumor cell lines as well as primary and metastatic human tumors, including lymphomas, carcinomas (colon, thyroid, mamma, bladder), and gliomas.

    Detection of abnormal regulation of CD44 splicing thus could be helpful in cancer diagnosis and disease evaluation.

    ab119518 detects all circulating CD44 isoforms comprising the sCD44var (v5) sequences. Determination of sCD44var (v5) will provide more detailed insight into different pathological modifications during cancer and other diseases.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similaritiesContains 1 Link domain.
  • DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    modifications    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P16070 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CD44CD44 antigenCD44 molecule
        CD44_HUMANCDw44Cell surface glycoprotein CD44Chondroitin sulfate proteoglycan 8CSPG8ECMR-IIIEpicanExtracellular matrix receptor IIIGP90 lymphocyte homing/adhesion receptorHCELLHematopoietic cell E and L selectin ligandHeparan sulfate proteoglycanHermes antigenHoming function and Indian blood group systemHUTCH-IHyaluronate receptorINIndian blood groupLHRMC56MDU2MDU3MIC4PGP-1PGP-IPgp1Phagocytic glycoprotein 1Phagocytic glycoprotein I
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119518 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      CD44var (v5) Human ELISA kit (with color giving dyes) images

      • Sandwich ELISA - CD44var (v5) Human ELISA kit (with color giving dyes) (ab119518)
        Sandwich ELISA - CD44var (v5) Human ELISA kit (with color giving dyes) (ab119518)
        Representative standard curve using ab119518. Human sCD44var (v5) was diluted in serial 2-fold steps in Sample Diluent.

      Protocols

      References for CD44var (v5) Human ELISA kit (with color giving dyes) (ab119518)

      ab119518 has not yet been referenced specifically in any publications.

      Publishing research using ab119518 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"