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CD44var (v6) Human ELISA kit (with color giving dyes) (ab119519)

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Overview

  • Product nameCD44var (v6) Human ELISA kit (with color giving dyes)
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 4.2%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    0.05 ng/ml
  • Range
    0.32 ng/ml - 10 ng/ml
  • Recovery

    106 %

    Sample specific recovery
    Sample type Average % Range
    Serum 106 89% - 115%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab119519 CD44var (v6) Human ELISA kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD44var (v6). ab119519 is for research use only. Not for diagnostic or therapeutic procedures.

    Alternative splicing of CD44 mRNA gives rise to the different isoforms, containing inserts of varying sizes in the extracellular domain of the molecule (exons v2-v10). All CD44 isoforms are variably glycosylated. In contrast to standard CD44 (CD44std) which is almost ubiquitously expressed, the variety of CD44 isoforms (CD44var) have a much more restricted distribution, e.g., on activated lymphocytes and macrophages (exon v6).

    ab119519 detects all circulating CD44 isoforms comprising the sCD44var (v6) sequences.

    Determination of sCD44var (v6) will provide more detailed insight into different pathological modifications during cancer and other diseases.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similaritiesContains 1 Link domain.
  • DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    modifications    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P16070 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CD44CD44 antigenCD44 molecule
        CD44_HUMANCDw44Cell surface glycoprotein CD44Chondroitin sulfate proteoglycan 8CSPG8ECMR-IIIEpicanExtracellular matrix receptor IIIGP90 lymphocyte homing/adhesion receptorHematopoietic cell E and L selectin ligandHeparan sulfate proteoglycanHermes antigenHoming function and Indian blood group systemHUTCH-IHyaluronate receptorINIndian blood groupLHRMC56MDU2MDU3MIC4PGP-1PGP-IPgp1Phagocytic glycoprotein 1Phagocytic glycoprotein I
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119519 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      CD44var (v6) Human ELISA kit (with color giving dyes) images

      • Sandwich ELISA - CD44var (v6) Human ELISA kit (with color giving dyes) (ab119519)
        Sandwich ELISA - CD44var (v6) Human ELISA kit (with color giving dyes) (ab119519)
        Representative standard curve using ab119519. Human sCD44var (v6) was diluted in serial 2-fold steps in Sample Diluent.

      Protocols

      References for CD44var (v6) Human ELISA kit (with color giving dyes) (ab119519)

      ab119519 has not yet been referenced specifically in any publications.

      Publishing research using ab119519 ? Please let us know so that we can cite the reference in this datasheet

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      I am looking at your ELISA detection kit for CD44(v6) - product #AB119519. I am looking into an ELISA based method of examining changes in phosphorylation of v6 in a proprietary system, and though your kit might be a good start. My question is this - the ...

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      Thank you for contacting us.

      After reading the protocol I am sure that the whole plate is coated with the antibody as all the reagents are designed to be sufficient for 12 stripes. As mentioned in the protocol, the 32 wells are just an example....

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"