 image](#CD45-Primary-antibodies-ab8216-1.jpg)
![Flow Cytometry - CD45 antibody [MEM-28] (ab8216) image](#CD45-Primary-antibodies-ab8216-2.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Adaptive Immunity >> B Cells >> CD
Overview
Product name
Anti-CD45 antibody [MEM-28]
See all CD45 products (76) ...
Description
Mouse monoclonal [MEM-28] to CD45
Specificity
Human CD45 antigen (LCA). This antibody reacts with all alternative forms of CD45.
Tested applications
Flow Cyt, IP, IHC-P, WB, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Tissue / cell preparation (Human). (Thymocytes and T lymphocytes).
Positive control
Jurkat human leukemia T-cell lysate; Kg-1a human leukemia cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
MEM-28
Isotype
IgG1
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Neuroscience >> Cell Type Marker >> Glia marker >> Microglia marker
Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Immunology >> Cell Type Markers >> CD >> Non-lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> B Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells. ((Recognizes an extracellular epitope).)
IP: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. Antigen retrieval is not essential but may optimise staining.
WB: Use a concentration of 2 µg/ml(Prepare and run samples in non-reducing conditions.)
ICC: Use at an assay dependent concentration.
Target
Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
Domain
The first PTPase domain interacts with SKAP1.
Post-translational
modifications
Heavily N- and O-glycosylated.
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
Target information above from: UniProt accessionP08575
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
Anti-CD45 antibody [MEM-28] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CD45 antibody [MEM-28](ab8216)
](/ps/datasheet/images/8/ab8216/CD45-Primary-antibodies-ab8216-1.jpg)
Ab8216 staining human normal tonsil tissue. Staining is localised to cellular membranes.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Flow Cytometry - CD45 antibody [MEM-28] (ab8216)
![Flow Cytometry - CD45 antibody [MEM-28] (ab8216)](/ps/datasheet/images/8/ab8216/CD45-Primary-antibodies-ab8216-2.jpg)
Overlay histogram showing peripheral blood lymphocytes stained with ab8216 (red line). The cells were incubated with the antibody (ab8216, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
References for Anti-CD45 antibody [MEM-28] (ab8216)
This product has been referenced in:
- Raymond ADet al. HIV Type 1 Nef is released from infected cells in CD45(+) microvesicles and is present in the plasma of HIV-infected individuals. AIDS Res Hum Retroviruses 27:167-78 (2011). WB; Human.Read more (PubMed: 20964480) »
- Ali SAet al. Genetic characterization of HIV type 1 Nef-induced vesicle secretion. AIDS Res Hum Retroviruses 26:173-92 (2010). WB; Human.Read more (PubMed: 20156100) »
See all 7 publications for this product
Publishing research using ab8216? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"