CD45 antibody [MEM-28] (ab8216)

Product Name 

CD45 antibody [MEM-28]

Product type 

Primary antibodies

Description 

Mouse monoclonal [MEM-28] to CD45

Immunogen 

Tissue / cell preparation (Human). (Thymocytes and T lymphocytes).

Reacts with 
(species key)

Hu

Specificity 

Human CD45 antigen (LCA). This antibody reacts with all alternative forms of CD45.

Tested applications 
(see key)

Flow Cyt, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution. Recognizes an extracellular epitope.
IHC-P: Use at an assay dependent dilution. Antigen retrieval is not essential but may optimise staining.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 µg/ml. Prepare and run samples in non-reducing conditions.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat human leukemia T-cell lysate; Kg-1a human leukemia cell lysate

Cellular localization 

Type I membrane protein.

Research areas 

Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Neuroscience >> Cell Type Marker >> Glia marker >> Microglia marker
Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Immunology >> Cell Type Markers >> CD >> Non-lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> B Cells >> CD

Relevance 

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

MEM-28

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for CD45 antibody [MEM-28] images, references, products related to ab8216 and other tools.

CD45 antibody [MEM-28] images:

References for CD45 antibody [MEM-28] (ab8216)

  This product has been used in: (two most recent references)

Goodman WA et al. IL-6 signaling in psoriasis prevents immune suppression by regulatory T cells. J Immunol 183:3170-6 (2009). IHC-P; Human. PubMed: 19648274

Kirchberger S et al. The cytoplasmic tail of CD45 is released from activated phagocytes and can act as an inhibitory messenger for T cells. Blood : (2008). PubMed: 18511809

See all 6 publication references for this product.


If you publish research using ab8216 please let us know so that we can cite the reference on this datasheet.

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