Anti-CD45 antibody (ab10559)

A customer needs a CD 45 antibody to be used in Immunoprecipitation. He wanted AB10559, which is not suitable for IP (only WB, Flow, ICC and IHC-P)
I checked the website and suggested AB106478.

Leonard has the following reservation and question:

The reason I was hoping that I could use ab10559 is because the immunogen is a peptide from the intracellular domain of CD45 and this is important for what we want to do. I notice that the info on ab106478 says that the immunogen is T lymphocytes. Can you possibly tell me what epitope the monoclonal goes for on CD45?

Please advise! If this antibody is not suitable, do you have another suggestion?

Thank you in advance for your help.

Regards

ANSWER:

Thank you for contacting us.

I am sorry we do not have epitope details of ab106478, at the moment. We will be updating the datasheet when this information will be available.

I can however recommend using antibody ab10559 in IP, although this hasn't been tested however this antibody will work. If the customer is interested in testing this Ab IP, I can happily provide Abreview testing discount. Full details can found by clicking the following link;

http://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Recommended compatible secondary antibody;

ab97051; http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-ab97051.html

We are giving away free Molecular Weight Marker Samples; please check

http://www.abcam.com/index.html?pageconfig=resource&rid=14769&utm_campaign=Brand.Giveaway&utm_source=Abcam.Brand&utm_medium=Email&utm_term=webbanner-proteinladder&intGoUser=6184200

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I would like to test ab10559 and ab64409 in samples.

ANSWER:

I am very pleased to hear you would like to accept our offer and test ab10559 and ab64409 in samples.This code will give you 1 freeprimary antibodybefore the expiration date for each discount code. To redeem this offer, please submit an Abreview for and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Dear Sir/Madam Please kindly recommend the abcam slide control for IHC-P application for this antibodies. 1. Anti-ErbB 2 antibody (ab2428) 2. Anti-Progesterone Receptor antibody [SP2] (ab16661) 3. Anti-Estrogen Receptor antibody [SP1] (ab16660) 4. Anti-Myeloperoxidase antibody (ab45977) 5. Anti-TdT antibody (ab85148) 6. Anti-TMEM16A antibody (ab53212) 7. Anti-NSE antibody [EPR3377] (ab79757) 8. Anti-CD23 antibody [EPR3617(IHC)] (ab92495) 9. Anti-CD10 antibody [56C6] (ab951) 10. Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) 11. Anti-CD79a antibody [HM47/A9] (ab3121) 12. Anti-CD45RO antibody [UCHL-1] (ab86080) 13. Anti-CD45 antibody (ab10559) 14. Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) 15. Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Best regards

ANSWER:

Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Human breast cancer 2.Anti-Progesterone Receptor antibody [SP2] (ab16661) Human breast carcinoma 3.Anti-Estrogen Receptor antibody [SP1] (ab16660) Human breast carcinoma 4.Anti-Myeloperoxidase antibody (ab45977) Human spleen tissue 5.Anti-TdT antibody (ab85148) Human testis 6.Anti-TMEM16A antibody (ab53212) Human liver 7.Anti-NSE antibody [EPR3377] (ab79757) Human brain tissue 8.Anti-CD23 antibody [EPR3617(IHC)] (ab92495) Human tonsil 9.Anti-CD10 antibody [56C6] (ab951) Lymph node tissue or human breast microepithelium 10.Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) Murine melanoma metastasis tissue 11.Anti-CD79a antibody [HM47/A9] (ab3121) Human Lymphoid tissue, tonsil 12.Anti-CD45RO antibody [UCHL-1] (ab86080) Human Tonsil, Thymus 13.Anti-CD45 antibody (ab10559) Human Tonsil tissue 14.Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) Human Hodgekins lymphoma tissue 15.Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Human Hodgekins lymphoma tissue Human spleen I am providing a list ofslides that we sell which I hope will be helpful to you. I can recommend to review the online datasheet for more details. Human brain: ab4305Brain (human): hippocampus normal tissue slides http://www.abcam.com/index.html?datasheet=4305 (or use the following: http://www.abcam.com/index.html?datasheet=4305). Human lymph node: ab4350Lymph node (human) normal tissue slides IHC-P, ISH http://www.abcam.com/index.html?datasheet=4350 (or use the following: http://www.abcam.com/index.html?datasheet=4350). Human testis: ab4373Testis (human) normal tissue slides IHC-P, ISH http://www.abcam.com/index.html?datasheet=4373 (or use the following: http://www.abcam.com/index.html?datasheet=4373). Human liver: ab4348Liver (human) normal tissue slides IHC-P, ISH http://www.abcam.com/index.html?datasheet=4348 (or use the following: http://www.abcam.com/index.html?datasheet=4348). Human spleen: ab4372Spleen (human) normal tissue slides IHC-P, ISH http://www.abcam.com/index.html?datasheet=4372 (or use the following: http://www.abcam.com/index.html?datasheet=4372). I am sorry we do not have slides forthe following: Human tonsil Hodgekins lymphoma tissue Mouse melenoma tissue I hope this will be helpful to you. If you have any further questions,please do not hesitate to contact us.

 Do you have the product number and price for the blocking peptide for ab103404? We are going to send you an order on that product and on some other products when we get that information.

Kind regards

ANSWER:

Thank you for contacting us.

I apologize for the delay. The catalogue number of blocking peptide is ab116167.

The price is £110 for 100 ug size.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Dear Sir/Madam

1)     We are doing western blot experiments for quantification. I read in the antibody guides that it would not be an advantage using polyclonals in quantification experiments.

Is it absolutely not recommended using polyclonals when we are quantifying?

2)     Can you please recommend a positive control and a blocking peptide for IL7Ralpha (ab95023)

3)     Can you please recommend a blocking peptide for CCR7 (ab103404)

4)     What antibody for TLR-4 and CD45 do you recommend?

Experiment: Western blot on human blood samples (mostly white blood cells (but contaminated with plasma and red blood cells)). We are going to quantify the bands. We would also appreciate recommendations for positive controls and for blocking peptides fore these antibodies.

Kind regards

ANSWER:

Thank you for contacting us.

The positive controls for ab95023 are cell lysates of A-431, U2OS, U251 MG, Hela cell etc. Please click the following to see the positive control tissue lysates;

http://www.proteinatlas.org/ENSG00000168685

We unfortunately cannot offer blocking peptide for this antibody; the peptide is not commercially available.

We can certainly offer a blocking peptide for ab103404; let me know if you are interested in buying this we will then publish this product as special request.

TLR4 antibody:-

We can recommend ab83444; http://www.abcam.com/tlr4-antibody-ab83444.html

The blocking peptide for this is ab93304; http://www.abcam.com/TLR4-peptide-ab93304.html

The positive control lysates would be Human, Mouse and Rat small intestine; NIH3T3; EL4 etc.

CD45 antibody and peptide would be:-

ab10559; http://www.abcam.com/CD45-antibody-ab10559.html and ab17550; http://www.abcam.com/CD45-peptide-ab17550.html

The positive control will be Jurkat, KM-H2, or human tonsil tissue lysates. Please also check human tissue Atlas for more information about positive control.

Polyclonal antibodies can be used for protein quantifications also however because monoclonal antibodies binds more specifically to particular proteins so monoclonal antibodies are recommended more than polyclonal antibodies. The choice antibodies are depends on the type of application used e.g. in sandwich ELISA monoclonal is best.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.