 image](#CD45RO-Primary-antibodies-ab23-1.jpg)
![Western blot - Anti-CD45RO antibody [UCH-L1] (ab23) image](#CD45RO-Primary-antibodies-ab23-5.jpg)
![Flow Cytometry - Anti-CD45RO antibody [UCH-L1] (ab23) image](#CD45RO-Primary-antibodies-ab23-4.jpg)
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Product name
Anti-CD45RO antibody [UCH-L1]
See all CD45RO products (9) ...
Description
Mouse monoclonal [UCH-L1] to CD45RO
Specificity
UCH-L1 may identify abnormalities of functional T-cell subsets in immune disorders and can be used in tumour diagnosis by FACS or immunohistology. Very useful in studies of human T cell function and ontogeny.
Tested applications
Flow Cyt, IP, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.
Positive control
This antibody gave a positive signal in human tonsil and thymus tissue lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
UCH-L1
Myeloma
P3-NS1/1-Ag4-1
Isotype
IgG2a
Research areas
Immunology >> Cell Type Markers >> CD >> Non-lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> B Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells.
IP: Use at an assay dependent dilution.
WB: Use a concentration of 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
Domain
The first PTPase domain interacts with SKAP1.
Post-translational
modifications
Heavily N- and O-glycosylated.
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
Target information above from: UniProt accessionP08575
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
Anti-CD45RO antibody [UCH-L1] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CD45RO antibody [UCH-L1](ab23)
](/ps/datasheet/images/0/ab23/CD45RO-Primary-antibodies-ab23-1.jpg)
ab23 (1µg/ml) staining CD45RO in human tonsil.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot - Anti-CD45RO antibody [UCH-L1] (ab23)
![Western blot - Anti-CD45RO antibody [UCH-L1] (ab23)](/ps/datasheet/images/0/ab23/CD45RO-Primary-antibodies-ab23-5.jpg)
All lanes : Anti-CD45RO antibody [UCH-L1] (ab23) at 5 µg/ml
Lane 1 :
Lane 2 :
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 131 kDa
Observed band size : 180 kDa (why is the actual band size different from the predicted?)
Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
CD45RO contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Flow Cytometry - Anti-CD45RO antibody [UCH-L1] (ab23)
![Flow Cytometry - Anti-CD45RO antibody [UCH-L1] (ab23)](/ps/datasheet/images/0/ab23/CD45RO-Primary-antibodies-ab23-4.jpg)
Overlay histogram showing HeLa cells stained with ab23 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References for Anti-CD45RO antibody [UCH-L1] (ab23)
This product has been referenced in:
- Smith SH et al. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology 58:63-70 (1986).Read more (PubMed: 2423439) »
- Norton AJ et al. Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol 39:399-405 (1986). IHC-Fr, IHC-P.Read more (PubMed: 2422219) »
See all 2 publications for this product
Publishing research using ab23? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"