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Product Name
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CD46 antibody [MEM-258] (FITC)
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See all CD46 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [MEM-258] to CD46 (FITC)
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Immunogen
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Tissue / cell preparation: T cell line HPB-ALL (Human).
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Reacts with
(species key)
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Hu, Cow
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Specificity
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The antibody recognizes an epitope on SCR4 (the membrane proximal SCR)domain of CD46 antigen (membrane cofactor protein).
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Tested applications
(see key)
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Flow Cyt
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 20µl for 106 cells in a suspension or 100 µl of whole blood.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human blood cells.
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Cellular localization
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Type I membrane protein.
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Research areas
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Immunology >> Innate Immunity >> Complement >> Regulatory
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Relevance
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CD46 is one of the membrane complement regulatory proteins. CD46 possesses C3b-binding and factor I cofactor activities which play important roles in the regulation of the complement activation pathway. CD46 is widely distributed on blood cells, endothelial cells, epithelial cells and tumor cell lines. CD46 exists as many isoforms in a variety of tissues. The antigen has a broad distribution and is present on leukocytes, platelets, endothelial cells, epithelial cells and fibroblasts. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. It is expressed on thymocytes, B cells, monocytes, granulocytes, NK cells, platelets, epithelial and endothelial cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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MEM-258
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Isotype
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IgG1
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Purity
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>95% by SDS-PAGE
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Conjugation notes
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The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
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Storage buffer
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Preservative: 15mM Sodium Azide.
Constituents: 0.2% high grade, protease free BSA, PBS. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Size exclusion chromatography.
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Form
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Liquid
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Conjugation
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Fluorescein
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Storage instructions
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Store at +4°C in the dark. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD46 antibody [MEM-258] (FITC) is on this datasheet. But please do contact us if you would like any reassurance! |
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References for CD46 antibody [MEM-258] (FITC) (ab28068)
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This product has been used in:
Jing Y et al. Tumor and vascular targeting of a novel oncolytic measles virus retargeted against the urokinase receptor. Cancer Res 69:1459-68 (2009). Flow Cyt. PubMed: 19208845
If you publish research using ab28068 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD46
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CD46 antibody [MEM-258] (FITC) - more information
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Customer reviews (feedback) regarding CD46 antibody [MEM-258] (FITC) |
Customer FAQs regarding CD46 antibody [MEM-258] (FITC) |
Protocols for CD46 antibody [MEM-258] (FITC) |
Price and availability of products related to CD46 antibody [MEM-258] (FITC) |
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CD46 antibody [MEM-258] (FITC) - other tools:
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