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Does this antibody recognize all CD46 isoforms? |
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ANSWER: |
The antibody MEM-258 reacts with several bands in Western Blotting, however, detailed study was not perform if antibody recognizes all CD46 isoforms. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-CD46 antibody [MEM-258] (ab789) at 1 µg/ml
Lane 1 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 :
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 44 kDa
Observed band size : 44 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
ab789 detecting CD46 in Human THP-1 cells by Flow Cytometry. Cells were 1.25X10^5 cells/sample in 2% FBS/0.05% NaN3/PBS. The primary antibody was used at a concentration of 0.5 µg/cells and incubated with the sample for 15 minutes at 22ºC. The secondary antibody was a FITC-conjugated F(ab') Goat anti-Mouse IgG(H+L) polyclonal antibody used at a concentration of 20 µg/ml.
This image is courtesy of an anonymous Abreview.
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