|
|
|
|
Immunocytochemistry/ Immunofluorescence - CD47 antibody [B6H12.2] (ab3283)
![Immunocytochemistry/ Immunofluorescence - CD47 antibody [B6H12.2] (ab3283)](/ps/datasheet/images/ab3283_1.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
CD47 antibody [B6H12.2]
|
| |
See all CD47 antibodies (5)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [B6H12.2] to CD47
|
|
Immunogen
|
Intact CD47 purified from placenta.
|
|
Reacts with
(species key)
|
Hu
|
|
Epitope
|
Ig domain.
|
|
Tested applications
(see key)
|
Flow Cyt, FuncS, ICC/IF, IP
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
Flow Cytometry / Immunofluorescence (methanol fixed only) / Functional studies: assay dependent.
Immunoprecipitation (native, with protein G): use at a concentration of 2µg / mg protein lysate.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
This antibody blocks the binding of SIRP alpha (Yoshida, et al.; Latour et al.) and inhibits in assays where CD47-integrin association is required.
|
|
Positive control
(see definition)
|
Tonsil.
|
|
Cellular localization
|
Cell membrane; multi pass membrane protein.
|
|
Research areas
|
Cancer >> Tumor immunology >> CD markers
Immunology >> Cell Type Markers >> CD >> Adhesion
|
|
Relevance
|
CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
B6H12.2
|
|
Isotype
|
IgG1
|
|
Light chain type
|
kappa
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.200 mg/ml
|
|
Storage instructions
|
Store at +4°C. Do not freeze.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD47 antibody [B6H12.2] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for CD47 antibody [B6H12.2] images, references, products related to ab3283 and other tools.
|
| |
CD47 antibody [B6H12.2] images:
|
 Immunocytochemistry/ Immunofluorescence - CD47 antibody [B6H12.2] (ab3283)
ab3283 at 1µg/ml staining CD47 from human OVCA3 cells by ICC. The cells were methanol fixed, blocked with 3% serum and incubated with the primary antibody for 30 minutes. A biotinylated horse anti-mouse IgG was used as the secondary antibody. This image is courtesy of an Abreview submitted by Mr Benjamin Misemer
See Abreview
|
| |
References for CD47 antibody [B6H12.2] (ab3283)
|
|
This product has been used in: (two most recent references)
Filla MS et al. Regulation of cross-linked actin network (CLAN) formation in human trabecular meshwork (HTM) cells by convergence of distinct beta1 and beta3 integrin pathways. Invest Ophthalmol Vis Sci 50:5723-31 (2009). FuncS; Human. PubMed: 19643963
Yoshida H et al. Interaction between Src homology 2 domain bearing protein tyrosine phosphatase substrate-1 and CD47 mediates the adhesion of human B lymphocytes to nonactivated endothelial cells. J Immunol 168:3213-20 (2002). PubMed: 11907074
See all 6 publication references for this product.
If you publish research using ab3283 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to CD47
|
| |
CD47 antibody [B6H12.2] - more information
|
Customer reviews (feedback) regarding CD47 antibody [B6H12.2] |
Customer FAQs regarding CD47 antibody [B6H12.2] |
Protocols for CD47 antibody [B6H12.2] |
Price and availability of products related to CD47 antibody [B6H12.2] |
| |
CD47 antibody [B6H12.2] - related products:
|
|
|
| |
CD47 antibody [B6H12.2] - other tools:
|
Contact Abcam with a Technical Enquiry about CD47 antibody [B6H12.2] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunocytochemistry/ Immunofluorescence - CD47 antibody [B6H12.2] (ab3283)](/index.html?datasheet=3283#ab3283_1.jpg)
