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Product Name
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CD47 antibody [MEM-122]
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See all CD47 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [MEM-122] to CD47
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Immunogen
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COS-7 cells transfected with human CD47.
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Reacts with
(species key)
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Hu, Mk, NHuPrm, Pig
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Specificity
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Integrin Associated Protein
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Tested applications
(see key)
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Flow Cyt, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
WB: 1/100. Use in non-reducing conditions.
This antibody causes platelet aggregation and ATP release from platelet-rich plasma but not from washed platelets, indicating a complement-dependent reaction.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell membrane; multi pass membrane protein.
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Research areas
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Cancer >> Tumor immunology >> CD markers
Immunology >> Cell Type Markers >> CD >> Adhesion
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Relevance
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CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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MEM-122
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Isotype
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IgM
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from ascites by precipitation methods and size-exclusion chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD47 antibody [MEM-122] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to CD47
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CD47 antibody [MEM-122] - more information
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