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Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-CD47 antibody [OX-101]
See all CD47 products (7) ...
Description
Mouse monoclonal [OX-101] to CD47
Specificity
Ab33852 recognises CD47 expressed primarily by lymphocytes and macrophages but also detected at high levels on brain endothelial cells.
Tested applications
Flow Cyt, IP, WB, Functional Studiesmore details
Cross reactivity
Reacts with
Rat
Immunogen
Tissue / cell preparation: Rat thymocytes
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Ab33852 was purified from tissue culture supernatant.
Primary antibody notes
OX-101 (this clone) recognises the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP).
Clonality
Monoclonal
Clone number
OX-101
Isotype
IgG1
Research areas
Cancer >> Tumor immunology >> CD markers
Immunology >> Cell Type Markers >> CD >> Adhesion
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
IP
WB
Functional Studies
Application notes
Flow Cyt: Use 10µl for 106 cells in 100µl.
FuncS: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 33 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
Tissue specificity
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ08722
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigenic surface determinant protein OA3 antibody
- CD 47 antibody
- CD47 antibody
see all
Database links
- Entrez Gene: 29364 Rat
- SwissProt: P97829 Rat
- Unigene: 7409 Rat
References for Anti-CD47 antibody [OX-101] (ab33852)
This product has been referenced in:
- Vernon-Wilson EFet al. CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRPalpha 1. Eur J Immunol 30:2130-7 (2000).Read more (PubMed: 10940903) »
See 1 publication for this product
Publishing research using ab33852? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"