CD5 + CD19 antibody [CRIS1 + CB19] (Dual color: FITC + PE) (ab1235)

Product Name 

CD5 + CD19 antibody [CRIS1 + CB19] (Dual color: FITC + PE)

Product type 

Primary antibodies

Description 

Mouse monoclonal [CRIS1 + CB19] to CD5 + CD19 (Dual color: FITC + PE)

Reacts with 
(species key)

Hu

Tested applications 
(see key)

Flow Cyt

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Counting and identification of peripheral blood T CD5+ lymphocytes (CD5+/CD19-) in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
Counting and identification of peripheral blood B lymphocytes (CD5-/CD19+ andCD5+/CD19+).
Counting and identification of peripheral blood B1 lymphocytes (CD5+/CD19+).
Characterization of CLL and lymphomas (CD5+/CD19+).

CD5(FITC) / CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

10µl ofCD5(FITC) / CD19 (PE) is sufficient for labelling 1x10⁶ cells.

Cellular localization 

Membrane; single-pass type I membrane protein.

Research areas 

Cancer >> Tumor biomarkers >> Tumor antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> B Cells >> CD

Relevance 

CD5 is a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most thymocytes and B lymphocytes subset (B-1a lymphocytes) and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.
The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor.
CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12% of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.

CD5 is a 67kDa human T-lymphocyte single-chain transmembrane glycoprotein. It is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. It also reacts with a subpopulation of activated B-cells.

CD19: This antigen (MW = 95kDa) is present on 10-15% of human peripheral blood lymphocytes andapproximately 60% of splenic lymphocytes. It is not expressed on T lymphocytes, granulocytes, activated T cells or monocytes. Moreover, CD19 is present on principal acute and chronic B cell leukemias and lymphomas.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

CRIS1 + CB19

Storage buffer 

PBS with 0.5% BSA and 0.1% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Conjugation 

Dual color: FITC + PE

Storage instructions 

Store at +4°C in the dark. Do not freeze.

Notes 

This product is a mixture of two clones:

CD5 = Clone:CRIS1, Isotype:IgG2a, Label:FITC
CD19 = Clone:CB19, Isotype:IgG1, Label:PE

Customer reviews (feedback) regarding CD5 + CD19 antibody [CRIS1 + CB19] (Dual color: FITC + PE)

Customer FAQs regarding CD5 + CD19 antibody [CRIS1 + CB19] (Dual color: FITC + PE)

Protocols for CD5 + CD19 antibody [CRIS1 + CB19] (Dual color: FITC + PE)

Price and availability of products related to CD5 + CD19 antibody [CRIS1 + CB19] (Dual color: FITC + PE)