CD5 + CD20 antibody [B-B8 (CD5) - B-H20 (CD20)] (Dual color: FITC + PE) (ab58897)

Product Name 

CD5 + CD20 antibody [B-B8 (CD5) - B-H20 (CD20)] (Dual color: FITC + PE)

Product type 

Primary antibodies

Description 

Mouse monoclonal [B-B8 (CD5) - B-H20 (CD20)] to CD5 + CD20 (Dual color: FITC + PE)

Immunogen 

Tissue / cell preparation (Human)

Reacts with 
(species key)

Hu

Specificity 

Clone B-B8 recognises CD5; a 67kD glycoprotein expressed by all mature T cells and by a subset of mature B cells.
Clone B-H20 recognises CD20 which is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells.

Tested applications 
(see key)

Flow Cyt

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use Neat: 20µl for 10⁶ cells or 100µl whole blood.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole blood: lymphocytes

Cellular localization 

Cell Membrane

Research areas 

Immunology >> Cell Type Markers >> CD >> Non-lineage
Immunology >> Adaptive Immunity >> B Cells >> CD

Relevance 

CD5 is a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most thymocytes and B lymphocytes subset (B-1a lymphocytes) and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.
The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor.
CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

B-B8 (CD5) - B-H20 (CD20)

Purity 

Ion Exchange Chromatography

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Conjugation 

Dual color: FITC + PE

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C. Do not freeze.

Notes 

This product is a mixture of two clones:

CD5 = Clone:B-B8, Isotype:IgG1, Label:FITC

CD20 = Clone:B-H20, Isotype:IgG2a, Label:PE

CD5 + CD20 antibody [B-B8 (CD5) - B-H20 (CD20)] (Dual color: FITC + PE) images:

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