CD5 antibody [4C7] (ab851)

Product Name 

CD5 antibody [4C7]

Product type 

Primary antibodies

Description 

Mouse monoclonal [4C7] to CD5

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
This antibody may be diluted to a titer of 1:50-1:100 in an ABC method.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.
Heat mediated antigen retrieval of formalin fixed paraffin embedded tissues may be required before immunostaining.

Positive control 
(see definition)

Mantle cell lymphoma

Cellular localization 

Type I membrane protein

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> B Cells >> CD

Relevance 

In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

4C7

Isotype 

IgG1

Light chain type 

kappa

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, Carrier protein, Van Gogh Yellow Diluent, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C.

References for CD5 antibody [4C7] (ab851)

  This product has been used in: (two most recent references)

Tateyama H et al. Immunoreactivity of a new CD5 antibody with normal epithelium and malignant tumors including thymic carcinoma. Am J Clin Pathol 111:235-40 (1999). PubMed: 9930146

Kaufmann O et al. Immunohistochemical detection of CD5 with monoclonal antibody 4C7 on paraffin sections. Am J Clin Pathol 108:669-73 (1997). PubMed: 9384449
If you publish research using ab851 please let us know so that we can cite the reference on this datasheet.

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